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Literature summary extracted from

  • Lolkema, J.S.; Kuiper, H.; ten Hoeve-Duurkens, R.H.; Robillard, G.T.
    Mannitol-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli: Physical size of enzyme IImtl and its domains IIBA and IIC in the active state (1993), Biochemistry, 32, 1396-1400.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.197 membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.197 38000
-
 1 * 38000 + x * 175000, domains IIBA and IIC, SDS-PAGE Escherichia coli
2.7.1.197 175000
-
 1 * 38000 + x * 175000, domains IIBA and IIC, SDS-PAGE Escherichia coli
2.7.1.197 315000
-
 globular protein solubilized in decylPEG detergent, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] Escherichia coli
-
 [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ir
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] Escherichia coli ML308-225
-
 [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.197 Escherichia coli
-
-
-
2.7.1.197 Escherichia coli ML308-225
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.197 hexylagarose column chromatography and TSK-250 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
 Escherichia coli [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ir
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
 Escherichia coli ML308-225 [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.1.197 ? 1 * 38000 + x * 175000, domains IIBA and IIC, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.197 mannitol-specific enzyme II
-
 Escherichia coli
2.7.1.197 mtlI
-
 Escherichia coli