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Literature summary extracted from

  • Hyakutake, M.; Tomizawa, S.; Mizuno, K.; Abe, H.; Tsuge, T.
    Alcoholytic cleavage of polyhydroxyalkanoate chains by class IV synthases induced by endogenous and exogenous ethanol (2014), Appl. Environ. Microbiol., 80, 1421-1429.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 expression in Escherichia coli Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Bacillus cereus D2Z0B8 and D2Z0B6 D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
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2.3.1.304 Bacillus cereus YB-4 D2Z0B8 and D2Z0B6 D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
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 Bacillus cereus [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
2.3.1.304 (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
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 Bacillus cereus YB-4 [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
2.3.1.304 additional information enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol Bacillus cereus ?
-
 ?
2.3.1.304 additional information enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol Bacillus cereus YB-4 ?
-
 ?

General Information

EC Number General Information Comment Organism
2.3.1.304 physiological function recombinant Escherichia coli expressing PHA synthase from Bacillus cereus shows a reduction of the molecular weight of PHA produced during the stationary phase of growth. Its carboxy end structure is capped by ethanol, as the result of alcoholytic cleavage of PHA chains by PhaRC induced by endogenous ethanol. This scission reaction is also induced by exogenous ethanol in both in vivo and in vitro assays. In addition, PhaRC has alcoholysis activity for PHA chains synthesized by other synthases Bacillus cereus