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Literature summary extracted from

  • Muheim, C.; Bakali, A.; Engstroem, O.; Wieslander, A.; Daley, D.O.; Widmalm, G.
    Identification of a fragment-based scaffold that inhibits the glycosyltransferase WaaG from Escherichia coli (2016), Antibiotics, 5, 10.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.B64 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate presence of 20 mM in assay, enhances activity Escherichia coli
2.4.1.B64 cardiolipin presence of 1 mM in assay, enhances activity Escherichia coli
2.4.1.B64 phosphatidylglycerol presence of 10 mM in assay, enhances activity Escherichia coli

Application

EC Number Application Comment Organism
2.4.1.B64 analysis activity assay for WaaG using 14C-labeled UDP-glucose and lipopolysaccharide purified from a WaaG deletion strain of Escherichia coli. Addition of the lipids phosphatidylglycerol and cardiolipin, as well as the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate increase activity Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B64 expression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.B64 4-(2-amino-1,3-thiazol-4-yl)phenol
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.B64 Mg2+ absolutely required, assay in presence of 25 mM MgCl2 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B64 Escherichia coli Q9R2L8
-
-
2.4.1.B64 Escherichia coli F470 Q9R2L8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B64 UDP-alpha-D-glucose + truncated lipopolysaccharide i.e. lipopolysaccharide isolated from a WaaG-deletion strain Escherichia coli UDP + glucosylated truncated lipopolysaccharide
-
 ?
2.4.1.B64 UDP-alpha-D-glucose + truncated lipopolysaccharide i.e. lipopolysaccharide isolated from a WaaG-deletion strain Escherichia coli F470 UDP + glucosylated truncated lipopolysaccharide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B64 WaaG
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.B64 1
-
 pH 7.5, 22°C Escherichia coli 4-(2-amino-1,3-thiazol-4-yl)phenol