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Literature summary extracted from

  • Safonova, T.N.; Mordkovich, N.N.; Veiko, V.P.; Okorokova, N.A.; Manuvera, V.A.; Dorovatovskii, P.V.; Popov, V.O.; Polyakov, K.M.
    Concerted action of two subunits of the functional dimer of Shewanella oneidensis MR-1 uridine phosphorylase derived from a comparison of the C212S mutant and the wild-type enzyme (2016), Acta Crystallogr. Sect. D, 72, 203-210.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3 mutant C212S is expressed in Escherichia coli JM110 cells Shewanella oneidensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 mutant enzyme C212S, hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Tris pH 8.5, and 25% (w/v) PEG 3350 Shewanella oneidensis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.3 C212S the mutation accounts for the lower affinity of the mutant for inorganic phosphate, while the affinity for uridine remains unchanged Shewanella oneidensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 0.23
-
 uridine wild type enzyme, at pH 7.4 and 37°C Shewanella oneidensis
2.4.2.3 0.24
-
 uridine mutant enzyme C212S, at pH 7.4 and 37°C Shewanella oneidensis
2.4.2.3 5.1
-
 phosphate wild type enzyme, at pH 7.4 and 37°C Shewanella oneidensis
2.4.2.3 16.1
-
 phosphate mutant enzyme C212S, at pH 7.4 and 37°C Shewanella oneidensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Shewanella oneidensis
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate Shewanella oneidensis MR-1 / ATCC 700550
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Shewanella oneidensis C7EWM3
-
-
2.4.2.3 Shewanella oneidensis MR-1 / ATCC 700550 C7EWM3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3
-
 Shewanella oneidensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 uridine + phosphate
-
 Shewanella oneidensis uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Shewanella oneidensis MR-1 / ATCC 700550 uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.3 homohexamer
-
 Shewanella oneidensis