EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.103 | expressed in Escherichia coli BL21(DE3) cells | Plasmodium knowlesi |
2.1.1.103 | expressed in Escherichia coli BL21(DE3) cells | Plasmodium vivax |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.103 | hanging drop vapor diffusion method, using 100 mM Na-acetate (pH 5.0), and 20% (w/v) PEG 3350 | Plasmodium knowlesi |
2.1.1.103 | sitting drop vapor diffusion method, using 200 mM NH4-formate and 20% (w/v) PEG 3350 | Plasmodium vivax |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.103 | amodiaquine | - |
Plasmodium knowlesi | |
2.1.1.103 | amodiaquine | - |
Plasmodium vivax | |
2.1.1.103 | Chloroquine | - |
Plasmodium knowlesi | |
2.1.1.103 | Chloroquine | - |
Plasmodium vivax | |
2.1.1.103 | NSC158011 | - |
Plasmodium knowlesi | |
2.1.1.103 | NSC158011 | - |
Plasmodium vivax |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.103 | 0.1 | - |
Ethanolamine phosphate | at pH 8.6 and 37°C | Plasmodium knowlesi | |
2.1.1.103 | 0.21 | - |
Ethanolamine phosphate | at pH 8.6 and 37°C | Plasmodium vivax | |
2.1.1.103 | 0.23 | - |
S-adenosyl-L-methionine | at pH 8.6 and 37°C | Plasmodium vivax | |
2.1.1.103 | 0.27 | - |
S-adenosyl-L-methionine | at pH 8.6 and 37°C | Plasmodium knowlesi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.103 | 31000 | - |
x * 31000, SDS-PAGE | Plasmodium knowlesi |
2.1.1.103 | 31000 | - |
x * 31000, SDS-PAGE | Plasmodium vivax |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.103 | S-adenosyl-L-methionine + ethanolamine phosphate | Plasmodium knowlesi | - |
S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? | |
2.1.1.103 | S-adenosyl-L-methionine + ethanolamine phosphate | Plasmodium vivax | - |
S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.103 | Plasmodium knowlesi | B3L8G9 | - |
- |
2.1.1.103 | Plasmodium vivax | A5K867 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.103 | nickel HisTrap column chromatography and Superdex 75PG gel filtration | Plasmodium knowlesi |
2.1.1.103 | nickel HisTrap column chromatography and Superdex 75PG gel filtration | Plasmodium vivax |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.103 | S-adenosyl-L-methionine + ethanolamine phosphate | - |
Plasmodium knowlesi | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? | |
2.1.1.103 | S-adenosyl-L-methionine + ethanolamine phosphate | - |
Plasmodium vivax | S-adenosyl-L-homocysteine + N-methylethanolamine phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.103 | ? | x * 31000, SDS-PAGE | Plasmodium knowlesi |
2.1.1.103 | ? | x * 31000, SDS-PAGE | Plasmodium vivax |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.103 | phosphoethanolamine methyltransferase | - |
Plasmodium knowlesi |
2.1.1.103 | phosphoethanolamine methyltransferase | - |
Plasmodium vivax |
2.1.1.103 | PMT | - |
Plasmodium knowlesi |
2.1.1.103 | PMT | - |
Plasmodium vivax |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.1.1.103 | 0.05 | - |
at pH 8.6 and 37°C | Plasmodium knowlesi | NSC158011 | |
2.1.1.103 | 0.099 | - |
at pH 8.6 and 37°C | Plasmodium vivax | NSC158011 | |
2.1.1.103 | 0.146 | - |
at pH 8.6 and 37°C | Plasmodium knowlesi | Chloroquine | |
2.1.1.103 | 0.146 | - |
at pH 8.6 and 37°C | Plasmodium vivax | Chloroquine | |
2.1.1.103 | 0.22 | - |
at pH 8.6 and 37°C | Plasmodium vivax | amodiaquine | |
2.1.1.103 | 0.398 | - |
at pH 8.6 and 37°C | Plasmodium knowlesi | amodiaquine |