EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.6 | additional information | no significant changes in enzyme activity are observed in the presence of 10 mM ATP, citrulline, and carbamoyl phosphate, respectively | Streptococcus pyogenes |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.2.2 | gene arcC, genetic organization in the arc operon, sequence comparisons, recombinant expression in Escherichia coli strain DH5alpha | Streptococcus pyogenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.2 | ATP | - |
Streptococcus pyogenes | |
2.7.2.2 | L-arginine | slight inhibition | Streptococcus pyogenes | |
2.7.2.2 | L-citrulline | - |
Streptococcus pyogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.2 | 0.65 | - |
Carbamoyl phosphate | recombinant enzyme, pH 6.5, 37°C | Streptococcus pyogenes | |
2.7.2.2 | 0.72 | - |
ADP | recombinant enzyme, pH 6.5, 37°C | Streptococcus pyogenes | |
3.5.3.6 | 1.33 | - |
L-arginine | at pH 6.5 and 37°C | Streptococcus pyogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.2 | Mg2+ | required | Streptococcus pyogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.2.2 | 34200 | - |
x * 34200, SDS-PAGE | Streptococcus pyogenes |
3.5.3.6 | 47200 | - |
x * 47200, SDS-PAGE | Streptococcus pyogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.2 | ADP + carbamoyl phosphate | Streptococcus pyogenes | - |
ATP + NH3 + CO2 | - |
r | |
2.7.2.2 | ATP + NH3 + CO2 | Streptococcus pyogenes | - |
ADP + carbamoyl phosphate | - |
r | |
3.5.3.6 | L-arginine + H2O | Streptococcus pyogenes | - |
L-citrulline + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.2 | Streptococcus pyogenes | A0A0H3BY05 | serotype M49, strain 591, gene arcC | - |
3.5.3.6 | Streptococcus pyogenes | B5XMC3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.2 | recombinant enzyme from Escherichia coli strain DH5alpha by affinity chromatography on StrepTactin resin | Streptococcus pyogenes |
3.5.3.6 | StrepTactin Sepharose column chromatography | Streptococcus pyogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.3.6 | 1.5 | - |
purified enzyme, at pH 6.5 and 37°C | Streptococcus pyogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.2 | ADP + carbamoyl phosphate | - |
Streptococcus pyogenes | ATP + NH3 + CO2 | - |
r | |
2.7.2.2 | ATP + NH3 + CO2 | - |
Streptococcus pyogenes | ADP + carbamoyl phosphate | - |
r | |
3.5.3.6 | L-arginine + H2O | - |
Streptococcus pyogenes | L-citrulline + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.2.2 | ? | x * 34200, SDS-PAGE | Streptococcus pyogenes |
3.5.3.6 | ? | x * 47200, SDS-PAGE | Streptococcus pyogenes |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.2.2 | ArcC | - |
Streptococcus pyogenes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.2 | 37 | - |
- |
Streptococcus pyogenes |
3.5.3.6 | 37 | - |
- |
Streptococcus pyogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.2 | 6.5 | - |
- |
Streptococcus pyogenes |
3.5.3.6 | 6.5 | - |
- |
Streptococcus pyogenes |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.2 | ADP | - |
Streptococcus pyogenes | |
2.7.2.2 | ATP | - |
Streptococcus pyogenes |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.2.2 | metabolism | the enzyme is part of the the arginine deiminase system, overview | Streptococcus pyogenes |