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Literature summary extracted from

  • Cullen, T.; Trent, M.
    A link between the assembly of flagella and lipooligosaccharide of the gram-negative bacterium Campylobacter jejuni (2010), Proc. Natl. Acad. Sci. USA, 107, 5160-5165.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.43 gene cj0256, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli K-12 strain W3110/EC01 Campylobacter jejuni

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.43 additional information generation of a mutant in Campylobacter jejuni 81-176 by interruption of cj0256 results in the absence of pEtN modifications on lipid A as well as FlgG Campylobacter jejuni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.43 periplasm
-
 Campylobacter jejuni
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.43 diacylphosphatidylethanolamine + flagellar rod protein FlgG Campylobacter jejuni
-
 ?
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A Campylobacter jejuni
-
 diacylglycerol + lipid A 1-(2-aminoethyl diphosphate)
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A Campylobacter jejuni
-
 diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
 ?
2.7.8.43 additional information Campylobacter jejuni the lipid A of Campylobacter jejuni is characterized by longer secondary acyl chains attached to the 2' and 3' positions of the molecule and by the addition of phosphoethanolamine to the phosphate groups attached at the 1 and 4' positions of the disaccharide backbone. The disaccharide backbone of Campylobacter jejuni lipid A is not composed solely of glucosamine residues, but can be replaced with the analogue 2,3-diamino-2,3-dideoxy-D-glucopyranose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.43 Campylobacter jejuni A0A3X8T8A0 gene cj0256
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.43 diacylphosphatidylethanolamine + flagellar rod protein FlgG
-
 Campylobacter jejuni ?
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + flagellar rod protein FlgG usage of recombinant C-terminally His6-tagged substrate protein Campylobacter jejuni ?
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A
-
 Campylobacter jejuni diacylglycerol + lipid A 1-(2-aminoethyl diphosphate)
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A
-
 Campylobacter jejuni diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
 ?
2.7.8.43 additional information the lipid A of Campylobacter jejuni is characterized by longer secondary acyl chains attached to the 2' and 3' positions of the molecule and by the addition of phosphoethanolamine to the phosphate groups attached at the 1 and 4' positions of the disaccharide backbone. The disaccharide backbone of Campylobacter jejuni lipid A is not composed solely of glucosamine residues, but can be replaced with the analogue 2,3-diamino-2,3-dideoxy-D-glucopyranose Campylobacter jejuni ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.43 Cj0256
-
 Campylobacter jejuni
2.7.8.43 pEtN transferase
-
 Campylobacter jejuni
2.7.8.43 phosphoethanolamine transferase
-
 Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.7.8.43 malfunction deletion of gene cj0256 results in the loss of phosphoethanolamine modification of lipid A and sensitivity to CAMPs, polymyxin B. Cj0256 mutants show decreased motility and greatly reduced flagella production. Interruption of cj0256 results in the absence of pEtN modifications on lipid A as well as FlgG. The cj0256 mutant showed a 20fold increase in sensitivity to the cationic antimicrobial peptide, polymyxin B, as well as a decrease in motility Campylobacter jejuni
2.7.8.43 physiological function the enzyme modifies two periplasmic targets, a membrane lipid A and a flagellar protein. It is required for efficient motility and flagella production Campylobacter jejuni