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Literature summary extracted from

  • Bialas, J.; Groettrup, M.; Aichem, A.
    Conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation (2015), PLoS ONE, 10, e0120329.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.45 HEK293 cells are transiently co-transfected with expression plasmids for HA-UBE1, the HA-tagged active site cysteine mutant UBE1 C632A, a His3-FLAG-tagged FAT10, a His3-FLAG-tagged FAT10 mutant lacking a diglycine motif at the C-terminus, or a His3-FLAG-tagged lysine-less FAT10 mutant Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.45 C632A generation of an active site cysteine mutant of HA-UBE1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.45 cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine Homo sapiens
-
 AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.45 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.2.1.45 additional information conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.45 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
-
 Homo sapiens AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.45 UBE1
-
 Homo sapiens
6.2.1.45 ubiquitin activating enzyme
-
 Homo sapiens
6.2.1.45 ubiquitin-activating enzyme 1
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
6.2.1.45 metabolism the ubiquitin E1 activating enzyme ubiquitin-activating enzyme 1 (UBE1) is a putative substrate of FAT10. The ubiquitin-like modifier HLA-F adjacent transcript 10 (FAT10) directly targets its substrates for proteasomal degradation by becoming covalently attached via its C-terminal diglycine motif to internal lysine residues of its substrate proteins. UBE1 and FAT10 formed a stable isopeptide linkage. The conjugation machinery consists of the bispecific E1 activating enzyme ubiquitin-like modifier activating enzyme 6 (UBA6), the likewise bispecific E2 conjugating enzyme UBA6-specific E2 enzyme 1 (USE1), and possibly E3 ligases, overview. UBE1 and FAT10 form a stable non-reducible conjugate, FAT10ylation of UBE1 depends on the diglycine motif of FAT10 Homo sapiens
6.2.1.45 additional information conjugation of the ubiquitin activating enzyme UBE1 with the ubiquitin-like modifier FAT10 targets it for proteasomal degradation. UBE1 uses Cys632 in the active site Homo sapiens
6.2.1.45 physiological function ubiquitin-activating enzyme 1 does not act as a second E1 activating enzyme for ubiquitin-like modifier HLA-F adjacent transcript but FAT10ylation of UBE1 leads to its proteasomal degradation Homo sapiens