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Literature summary extracted from

  • Mietkiewska, E.; Miles, R.; Wickramarathna, A.; Sahibollah, A.F.; Greer, M.S.; Chen, G.; Weselake, R.J.
    Combined transgenic expression of Punica granatum conjugase (FADX) and FAD2 desaturase in high linoleic acid Arabidopsis thaliana mutant leads to increased accumulation of punicic acid (2014), Planta, 240, 575-583.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.33 gene FADX, combined transgenic expression of Punica granatum conjugase (FADX) under the control of a seed-specific napin promoter and Arabidopsis thaliana FAD2 desaturase, EC 1.14.19.34, in high linoleic acid Arabidopsis thaliana fad3/fae1 mutant, via Agrobacterium tumefaciens cell strain GV3101 transformation, leads to increased accumulation of punicic acid, quantitative RT-PCR enzyme expression analysis. Arabidopsis thaliana seeds overexpressing PgFADX show accumulation of punicic acid up to 9.2% average in tandem lines (6.43% in lines expressing FADX alone), highest content in line NCJ-11 is 11.26%, phenotypes, overview. Overexpression of PgFADX affects the activity of the native Arabidopsis thaliana FAD2 desaturase, the relative AtFAD2 expression level is significantly reduced up to 99% Punica granatum
1.14.19.34 gene FAD2, combined transgenic expression of Punica granatum conjugase (FADX, EC 1.14.19.33) under the control of a seed-specific napin promoter and Arabidopsis thaliana FAD2 desaturase in high linoleic acid Arabidopsis thaliana fad3/fae1 mutant, via Agrobacterium tumefaciens cell strain GV3101 transformation, leads to increased accumulation of punicic acid, quantitative RT-PCR enzyme expression analysis Punica granatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.33 additional information Punica granatum linoleic acid serves as a precursor for punicic acid synthesis by the enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.33 Punica granatum Q84UB8 PgFADX; gene FADX
-
1.14.19.34 Punica granatum Q84VT2 gene FAD2
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.33 seed
-
 Punica granatum
-
1.14.19.34 seed
-
 Punica granatum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.33 additional information linoleic acid serves as a precursor for punicic acid synthesis by the enzyme Punica granatum ?
-
 ?
1.14.19.33 additional information the enzyme catalyzes the conversion of the DELTA12-double bond of linoleic acid (18:2DELTA9cis,12cis) into conjugated DELTA11trans and DELTA13cis-double bonds Punica granatum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.33 FADX
-
 Punica granatum
1.14.19.34 DELTA12-oleic acid desaturase
-
 Punica granatum
1.14.19.34 FAD2
-
 Punica granatum