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Literature summary extracted from

  • Wang, W.; Xi, H.; Bi, Q.; Hu, Y.; Zhang, Y.; Ni, M.
    Cloning, expression and characterization of D-aminoacylase from Achromobacter xylosoxidans subsp. denitrificans ATCC 15173 (2013), Microbiol. Res., 168, 360-366.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.81 synthesis the enzyme can be useful for industrial production of D-amino acids Achromobacter denitrificans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.81 gene ADdan, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) using pET-28a with a T7 promoter Achromobacter denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.81 Ca2+ low inhibition Achromobacter denitrificans
3.5.1.81 Cu2+
-
 Achromobacter denitrificans
3.5.1.81 Fe2+
-
 Achromobacter denitrificans
3.5.1.81 Hg2+
-
 Achromobacter denitrificans
3.5.1.81 Mn2+ low inhibition Achromobacter denitrificans
3.5.1.81 Ni2+ low inhibition Achromobacter denitrificans
3.5.1.81 Zn2+
-
 Achromobacter denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.81 additional information Mg2+ has no significant influence on the recombinant D-aminoacylase Achromobacter denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.81 52000
-
 x * 52000, recombinant His-tagged enzyme, SDS-PAGE Achromobacter denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.81 N-acyl-D-amino acid + H2O Achromobacter denitrificans
-
 a carboxylate + D-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.81 Achromobacter denitrificans S4T8N6 gene ADdan
-
3.5.1.81 Achromobacter denitrificans ATCC 15173 S4T8N6 gene ADdan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.81 recombinant His-tagged enzyme 8.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chhromatography and ultrafiltration to homogeneity Achromobacter denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.81 204
-
 purified recombinant enzyme, pH 7.8, 37°C Achromobacter denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.81 N-acetyl-D-alanine + H2O 16% of the activity with N-acetyl-D-methionine Achromobacter denitrificans acetate + D-alanine
-
 ?
3.5.1.81 N-acetyl-D-aspartate + H2O 25% of the activity with N-acetyl-D-methionine Achromobacter denitrificans acetate + D-aspartate
-
 ?
3.5.1.81 N-acetyl-D-aspartate + H2O 25% of the activity with N-acetyl-D-methionine Achromobacter denitrificans ATCC 15173 acetate + D-aspartate
-
 ?
3.5.1.81 N-acetyl-D-leucine + H2O 61% of the activity with N-acetyl-D-methionine Achromobacter denitrificans acetate + D-leucine
-
 ?
3.5.1.81 N-acetyl-D-leucine + H2O 61% of the activity with N-acetyl-D-methionine Achromobacter denitrificans ATCC 15173 acetate + D-leucine
-
 ?
3.5.1.81 N-acetyl-D-methionine + H2O best substrate Achromobacter denitrificans acetate + D-methionine
-
 ?
3.5.1.81 N-acetyl-D-methionine + H2O best substrate Achromobacter denitrificans ATCC 15173 acetate + D-methionine
-
 ?
3.5.1.81 N-acetyl-D-phenylalanine + H2O 54% of the activity with N-acetyl-D-methionine Achromobacter denitrificans acetate + D-phenylalanine
-
 ?
3.5.1.81 N-acetyl-D-phenylalanine + H2O 54% of the activity with N-acetyl-D-methionine Achromobacter denitrificans ATCC 15173 acetate + D-phenylalanine
-
 ?
3.5.1.81 N-acetyl-D-tryptophan + H2O 14% of the activity with N-acetyl-D-methionine Achromobacter denitrificans acetate + D-tryptophan
-
 ?
3.5.1.81 N-acetyl-D-tyrosine + H2O 5.0% of the activity with N-acetyl-D-methionine Achromobacter denitrificans acetate + D-tyrosine
-
 ?
3.5.1.81 N-acetyl-D-valine + H2O 8.0% of the activity with N-acetyl-D-methionine Achromobacter denitrificans acetate + D-valine
-
 ?
3.5.1.81 N-acetyl-D-valine + H2O 8.0% of the activity with N-acetyl-D-methionine Achromobacter denitrificans ATCC 15173 acetate + D-valine
-
 ?
3.5.1.81 N-acyl-D-amino acid + H2O
-
 Achromobacter denitrificans a carboxylate + D-amino acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.81 ? x * 52000, recombinant His-tagged enzyme, SDS-PAGE Achromobacter denitrificans

Synonyms

EC Number Synonyms Comment Organism
3.5.1.81 D-Aminoacylase
-
 Achromobacter denitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.81 50
-
 recombinant enzyme Achromobacter denitrificans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.81 25 50 activity range, recombinant enzyme, the activity decreased rapidly above 50°C Achromobacter denitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.81 45
-
 purified recombinant enzyme, pH 7.5, 30 min, 80% activity remaining Achromobacter denitrificans
3.5.1.81 50
-
 purified recombinant enzyme, pH 7.5, 30 min, 50% activity remaining Achromobacter denitrificans
3.5.1.81 55
-
 purified recombinant enzyme, pH 7.5, 30 min, inactivation Achromobacter denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.81 8
-
 recombinant enzyme Achromobacter denitrificans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.81 6.5 8.5 purified recombinant enzyme, 37°C, 30 min, stable at Achromobacter denitrificans