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Literature summary extracted from

  • Zargar, A.; Quan, D.N.; Emamian, M.; Tsao, C.Y.; Wu, H.C.; Virgile, C.R.; Bentley, W.E.
    Rational design of controller cells to manipulate protein and phenotype expression (2015), Metab. Eng., 30, 61-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.189 gene lsrK, overexpression in Escherichia coli strain LW12 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.189 intracellular
-
 Escherichia coli 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.189 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.189 ATP + autoinducer-2 Escherichia coli i.e. AI-2 ADP + phosphorylated autoinducer-2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.189 Escherichia coli
-
 W3110, gene lsrK
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.189 ATP + autoinducer-2 i.e. AI-2 Escherichia coli ADP + phosphorylated autoinducer-2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.189 AI-2 kinase
-
 Escherichia coli
2.7.1.189 Lsr kinase
-
 Escherichia coli
2.7.1.189 lsrK
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.189 ATP
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.1.189 metabolism model-based design, construction, and characterization of controller cells to modulate the external AI-2 environment, the cells are designed via the compartmentalization of different aspects of AI-2 processing: uptake (lsrACDB), phosphorylation (lsrK), and degradation (lsrFG), mathematical model overview Escherichia coli
2.7.1.189 physiological function the enzyme is responsible for the phosphorylation of autoinducer 2, and thereby attenuating cell-cell communication among bacterial populations. The bacterial signal molecule autoinducer-2 is secreted as a quorum sensing signal. The enzyme LsrK increases the net uptake rate by limiting secretion of AI-2 back into the extracellular environment. LsrK-mediated phosphorylation of AI-2 blocks export back to the extracellular milieu so that accumulated AI-2P binds the regulatory protein LsrR Escherichia coli