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Literature summary extracted from

  • Kuempornsin, K.; Kotanan, N.; Chobson, P.; Kochakarn, T.; Jirawatcharadech, P.; Jaru-ampornpan, P.; Yuthavong, Y.; Chookajorn, T.
    Biochemical and functional characterization of Plasmodium falciparum GTP cyclohydrolase I (2014), Malar. J., 13, 150.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.16 expressed in Escherichia coli BL21(DE3)RIL cells Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.16 H279S inactive Plasmodium falciparum
3.5.4.16 N88Y the mutant shows about wild type activity Plasmodium falciparum
3.5.4.16 R230K the mutant shows about wild type activity Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.16 8-oxo-GTP competitive inhibitor Plasmodium falciparum
3.5.4.16 KCl high salt (1 M KCl) diminishes the activity of the enzyme Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.16 0.0126
-
 GTP at pH 7.8 and 37°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.16 GTP + H2O Plasmodium falciparum
-
 formate + 7,8-dihydroneopterin 3'-triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.16 Plasmodium falciparum O97071
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.16 Ni2+-Sepharose column chromatography, and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.16 GTP + H2O
-
 Plasmodium falciparum formate + 7,8-dihydroneopterin 3'-triphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.16 GCH1
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.16 50
-
 the enzyme activity is improved by the rise in temperature up to 50°C Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.16 0.039
-
 GTP at pH 7.8 and 37°C Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.16 9
-
-
 Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.16 7 11
-
 Plasmodium falciparum