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Literature summary extracted from

  • Ryu, M.; Lee, E.
    Saccharification of alginate by using exolytic oligoalginate lyase from marine bacterium Sphingomonas sp. MJ-3 (2011), J. Ind. Eng. Chem., 17, 853-858.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.2.26 synthesis saccharification of alginate into alginate monosaccharides. Unsaturated monosaccharides up to 3.3 mg/ml are successfully prepared from 1% (w/v) alginate by using the recombinant oligoalginate lyase of Sphingomonas sp. MJ-3 Sphingomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.26 expression in Escherichia coli Sphingomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.26 Cu2+ 1 mM, 5% residual activity Sphingomonas sp.
4.2.2.26 Hg2+ 1 mM, no residual activity Sphingomonas sp.
4.2.2.26 Zn2+ 1 mM, 30% residual activity Sphingomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.26 KCl 1 mM, 120% of initial activity Sphingomonas sp.
4.2.2.26 NaCl 1 mM, 115% of initial activity Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.26 79900
-
 x * 79900, calculated Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.26 Sphingomonas sp. G1EH67
-
-
4.2.2.26 Sphingomonas sp. MJ-3 G1EH67
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.26 alginate
-
 Sphingomonas sp. unsaturated algino-monosaccharides alginate substrate is almost completely depolymerized into monosaccharides ?
4.2.2.26 alginate
-
 Sphingomonas sp. MJ-3 unsaturated algino-monosaccharides alginate substrate is almost completely depolymerized into monosaccharides ?

Subunits

EC Number Subunits Comment Organism
4.2.2.26 ? x * 79900, calculated Sphingomonas sp.