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HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples: evidence for substrate inhibition kinetics

Patel, M.; Vasaya, M.M.; Asker, D.; Parsons, R.B.; J. Chromatogr. B 921-922, 87-95 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.1
imipramine
slightly but significantly activates the mouse liver NNMT enzyme and also changes the kinetic parameters Vmax, Km and Ki
Mus musculus
Application
EC Number
Application
Commentary
Organism
2.1.1.1
medicine
inhibition of NNMT activity is a promising therapeutic target for cancer therapy
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.1
nicotinamide
the enzyme shows substrate inhibition kinetics
Homo sapiens
2.1.1.1
nicotinamide
the enzyme shows substrate inhibition kinetics
Mus musculus
2.1.1.1
nicotinamide
the enzyme shows substrate inhibition kinetics
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.1
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
2.1.1.1
additional information
-
additional information
Michaelis-Menten kinetics
Mus musculus
2.1.1.1
additional information
-
additional information
Michaelis-Menten kinetics, the Km value cannot be determined using Michaelis-Menten kinetic modelling
Oryctolagus cuniculus
2.1.1.1
1.02
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Mus musculus
2.1.1.1
1.36
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.1
cytosol
-
Homo sapiens
5829
-
2.1.1.1
cytosol
-
Mus musculus
5829
-
2.1.1.1
cytosol
-
Oryctolagus cuniculus
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
additional information
Mus musculus
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
?
-
-
-
2.1.1.1
additional information
Homo sapiens
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
?
-
-
-
2.1.1.1
additional information
Oryctolagus cuniculus
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
?
-
-
-
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Mus musculus
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Oryctolagus cuniculus
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Homo sapiens
-
-
-
2.1.1.1
Mus musculus
-
-
-
2.1.1.1
Oryctolagus cuniculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
liver
-
Homo sapiens
-
2.1.1.1
liver
-
Mus musculus
-
2.1.1.1
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
additional information
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
734368
Mus musculus
?
-
-
-
-
2.1.1.1
additional information
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
734368
Homo sapiens
?
-
-
-
-
2.1.1.1
additional information
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
734368
Oryctolagus cuniculus
?
-
-
-
-
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734368
Mus musculus
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734368
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734368
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
2.1.1.1
37
-
assay at
Mus musculus
2.1.1.1
37
-
assay at
Oryctolagus cuniculus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.1
1.02
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
7.2
-
assay at
Homo sapiens
2.1.1.1
7.2
-
assay at
Mus musculus
2.1.1.1
7.2
-
assay at
Oryctolagus cuniculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
2.1.1.1
S-adenosyl-L-methionine
-
Oryctolagus cuniculus
2.1.1.1
S-adenosyl-L-methionine
-
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.1
3.57
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Oryctolagus cuniculus
2.1.1.1
20.01
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Homo sapiens
2.1.1.1
50.64
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.1
imipramine
slightly but significantly activates the mouse liver NNMT enzyme and also changes the kinetic parameters Vmax, Km and Ki
Mus musculus
Application (protein specific)
EC Number
Application
Commentary
Organism
2.1.1.1
medicine
inhibition of NNMT activity is a promising therapeutic target for cancer therapy
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
2.1.1.1
S-adenosyl-L-methionine
-
Oryctolagus cuniculus
2.1.1.1
S-adenosyl-L-methionine
-
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.1
nicotinamide
the enzyme shows substrate inhibition kinetics
Homo sapiens
2.1.1.1
nicotinamide
the enzyme shows substrate inhibition kinetics
Mus musculus
2.1.1.1
nicotinamide
the enzyme shows substrate inhibition kinetics
Oryctolagus cuniculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.1
3.57
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Oryctolagus cuniculus
2.1.1.1
20.01
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Homo sapiens
2.1.1.1
50.64
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.1
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
2.1.1.1
additional information
-
additional information
Michaelis-Menten kinetics
Mus musculus
2.1.1.1
additional information
-
additional information
Michaelis-Menten kinetics, the Km value cannot be determined using Michaelis-Menten kinetic modelling
Oryctolagus cuniculus
2.1.1.1
1.02
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Mus musculus
2.1.1.1
1.36
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.1.1.1
cytosol
-
Homo sapiens
5829
-
2.1.1.1
cytosol
-
Mus musculus
5829
-
2.1.1.1
cytosol
-
Oryctolagus cuniculus
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
additional information
Mus musculus
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
?
-
-
-
2.1.1.1
additional information
Homo sapiens
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
?
-
-
-
2.1.1.1
additional information
Oryctolagus cuniculus
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
?
-
-
-
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Mus musculus
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Oryctolagus cuniculus
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
liver
-
Homo sapiens
-
2.1.1.1
liver
-
Mus musculus
-
2.1.1.1
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
additional information
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
734368
Mus musculus
?
-
-
-
-
2.1.1.1
additional information
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
734368
Homo sapiens
?
-
-
-
-
2.1.1.1
additional information
development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview
734368
Oryctolagus cuniculus
?
-
-
-
-
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734368
Mus musculus
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734368
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734368
Oryctolagus cuniculus
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
2.1.1.1
37
-
assay at
Mus musculus
2.1.1.1
37
-
assay at
Oryctolagus cuniculus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.1.1.1
1.02
-
nicotinamide
pH 7.2, 37°C, liver homogenate
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
7.2
-
assay at
Homo sapiens
2.1.1.1
7.2
-
assay at
Mus musculus
2.1.1.1
7.2
-
assay at
Oryctolagus cuniculus