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Literature summary extracted from
- Integrated development of an effective bioprocess for extracellular production of penicillin G acylase in Escherichia coli and its subsequent one-step purification (2012), J. Biotechnol., 161, 19-26.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.11 | gene pac, overexpression in Escherichia coli strain HB101 and secretion of the enzyme to the culture medium, method optimization, overview | Escherichia coli |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.11 | extracellular | - |
Escherichia coli | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | penicillin G + H2O | Escherichia coli | - |
phenylacetate + 6-aminopenicillanate | - |
? |  |
| 3.5.1.11 | penicillin G + H2O | Escherichia coli JE5505 | - |
phenylacetate + 6-aminopenicillanate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Escherichia coli | - |
- |
- |
| 3.5.1.11 | Escherichia coli JE5505 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.11 | recombinant extracellular enzyme 3.2fold from Escherichia coli strain HB101 culture supernatant by anion exchange chromatography, single-step purification | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 16.3 | - |
purified recombinant enzyme, pH 8.0, 37°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | penicillin G + H2O | - |
Escherichia coli | phenylacetate + 6-aminopenicillanate | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Escherichia coli JE5505 | phenylacetate + 6-aminopenicillanate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | PAC | - |
Escherichia coli |
| 3.5.1.11 | penicillin G acylase | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 37 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 8 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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