BRENDA - Enzyme Database

Revisiting the nucleotide and aminoglycoside substrate specificity of the bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia enzyme

Frase, H.; Toth, M.; Vakulenko, S.; J. Biol. Chem. 287, 43262-43269 (2012)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.190
butirosin A/B
-
Staphylococcus aureus
2.7.1.190
lividomycin A
-
Staphylococcus aureus
2.7.1.190
neomycin B
-
Staphylococcus aureus
2.7.1.190
paromomycin
-
Staphylococcus aureus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.0003
-
dibekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0008
-
sisomicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0008
-
arbekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0041
-
netilmicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.006
-
gentamicin C
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.033
-
amikacin
pH 7.0, 22°C
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.190
additional information
Staphylococcus aureus
GTP is the exclusive phosphate donor at intracellular nucleotide levels
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.190
Staphylococcus aureus
P0A0C1
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.1.190
GTP + amikacin
-
734204
Staphylococcus aureus
GDP + amikacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + arbekacin
-
734204
Staphylococcus aureus
GDP + arbekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + dibekacin
-
734204
Staphylococcus aureus
GDP + dibekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + gentamicin C
-
734204
Staphylococcus aureus
GDP + gentamicin C 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin A
-
734204
Staphylococcus aureus
GDP + kanamycin A 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin B
-
734204
Staphylococcus aureus
GDP + kanamycin B 2''-phosphate
-
-
-
?
2.7.1.190
GTP + netilmicin
-
734204
Staphylococcus aureus
GDP + netilmicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + sisomicin
-
734204
Staphylococcus aureus
GDP + sisomicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + tobramycin
-
734204
Staphylococcus aureus
GDP + tobramycin 2''-phosphate
-
-
-
?
2.7.1.190
additional information
GTP is the exclusive phosphate donor at intracellular nucleotide levels
734204
Staphylococcus aureus
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.1.190
bifunctional aminoglycoside acetyltransferase(6')-Ie/aminoglycoside phosphotransferase(2'')-Ia
-
Staphylococcus aureus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.11
-
tobramycin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.12
-
kanamycin B
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.15
-
dibekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.57
-
kanamycin A
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
2.3
-
arbekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
3
-
gentamicin C
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
3.7
-
amikacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
6.5
-
netilmicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
8.1
-
sisomicin
pH 7.0, 22°C
Staphylococcus aureus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.190
0.00026
-
neomycin B
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0053
-
paromomycin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.011
-
lividomycin A
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.14
-
butirosin A/B
pH 7.0, 22°C
Staphylococcus aureus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.190
butirosin A/B
-
Staphylococcus aureus
2.7.1.190
lividomycin A
-
Staphylococcus aureus
2.7.1.190
neomycin B
-
Staphylococcus aureus
2.7.1.190
paromomycin
-
Staphylococcus aureus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.190
0.00026
-
neomycin B
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0053
-
paromomycin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.011
-
lividomycin A
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.14
-
butirosin A/B
pH 7.0, 22°C
Staphylococcus aureus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.0003
-
dibekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0008
-
sisomicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0008
-
arbekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.0041
-
netilmicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.006
-
gentamicin C
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.033
-
amikacin
pH 7.0, 22°C
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.7.1.190
additional information
Staphylococcus aureus
GTP is the exclusive phosphate donor at intracellular nucleotide levels
?
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.1.190
GTP + amikacin
-
734204
Staphylococcus aureus
GDP + amikacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + arbekacin
-
734204
Staphylococcus aureus
GDP + arbekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + dibekacin
-
734204
Staphylococcus aureus
GDP + dibekacin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + gentamicin C
-
734204
Staphylococcus aureus
GDP + gentamicin C 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin A
-
734204
Staphylococcus aureus
GDP + kanamycin A 2''-phosphate
-
-
-
?
2.7.1.190
GTP + kanamycin B
-
734204
Staphylococcus aureus
GDP + kanamycin B 2''-phosphate
-
-
-
?
2.7.1.190
GTP + netilmicin
-
734204
Staphylococcus aureus
GDP + netilmicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + sisomicin
-
734204
Staphylococcus aureus
GDP + sisomicin 2''-phosphate
-
-
-
?
2.7.1.190
GTP + tobramycin
-
734204
Staphylococcus aureus
GDP + tobramycin 2''-phosphate
-
-
-
?
2.7.1.190
additional information
GTP is the exclusive phosphate donor at intracellular nucleotide levels
734204
Staphylococcus aureus
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.190
0.11
-
tobramycin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.12
-
kanamycin B
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.15
-
dibekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
0.57
-
kanamycin A
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
2.3
-
arbekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
3
-
gentamicin C
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
3.7
-
amikacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
6.5
-
netilmicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
8.1
-
sisomicin
pH 7.0, 22°C
Staphylococcus aureus
General Information
EC Number
General Information
Commentary
Organism
2.7.1.190
physiological function
enzyme phosphorylates 4,6-disubstituted aminoglycosides with high efficiency. Despite this proficiency, no resistance is conferred to some of these antibiotics by the enzyme in vivo. Phosphorylation of 4,5-disubstituted and atypical aminoglycosides are negligible and these antibiotics are not substrates. Instead, these aminoglycosides tend to stimulate an intrinsic GTPase activity of the enzyme
Staphylococcus aureus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.190
physiological function
enzyme phosphorylates 4,6-disubstituted aminoglycosides with high efficiency. Despite this proficiency, no resistance is conferred to some of these antibiotics by the enzyme in vivo. Phosphorylation of 4,5-disubstituted and atypical aminoglycosides are negligible and these antibiotics are not substrates. Instead, these aminoglycosides tend to stimulate an intrinsic GTPase activity of the enzyme
Staphylococcus aureus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.190
110
-
amikacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
500
-
dibekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
2900
-
arbekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
5000
-
gentamicin C
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
10000
-
sisomicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
16000
-
netilmicin
pH 7.0, 22°C
Staphylococcus aureus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.190
110
-
amikacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
500
-
dibekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
2900
-
arbekacin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
5000
-
gentamicin C
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
10000
-
sisomicin
pH 7.0, 22°C
Staphylococcus aureus
2.7.1.190
16000
-
netilmicin
pH 7.0, 22°C
Staphylococcus aureus