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Literature summary extracted from

  • Proffitt, K.D.; Virshup, D.M.
    Precise regulation of porcupine activity is required for physiological Wnt signaling (2012), J. Biol. Chem., 287, 34167-34178.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.250 gene PORCN, quantitative PCR enzyme expression analysis Mus musculus
2.3.1.250 gene PORCN, quantitative RT-PCR enzyme expression analysis. PORCN splice variants differ globally in signaling activity in HT-1080 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.250 H341A site-directed mutagenesis, catalytic active site mutant, Wnt signaling inactive mutant Mus musculus
2.3.1.250 additional information PORCN is deleted by adenoviral Cre-GFP excision in immortalized MEF cells, PORCN null cells cannot activate WNT3A Mus musculus
2.3.1.250 additional information zinc finger nuclease-mediated PORCN gene deletion in HT1080 cells. Mutation or skipping of PORCN exon 9 creates cells null for PORCN catalytic activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.250 endoplasmic reticulum membrane integral membrane protein, enzyme structure and topology model with cytoplasmic and endoplasmic reticulum luman parts, overview Homo sapiens 5789
-
2.3.1.250 endoplasmic reticulum membrane integral membrane protein, enzyme structure and topology model with cytoplasmic and endoplasmic reticulum luman parts, overview Mus musculus 5789
-
2.3.1.250 additional information the enzyme is predicted to palmitoylate Wnt either co-translationally or post-translationally, so that in either case, the catalytic site is likely to be in the membrane or in the lumen of the endoplasmic reticulum Homo sapiens
-
-
2.3.1.250 additional information the enzyme is predicted to palmitoylate Wnt either co-translationally or post-translationally, so that in either case, the catalytic site is likely to be in the membrane or in the lumen of the endoplasmic reticulum Mus musculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Homo sapiens the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Mus musculus the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Homo sapiens
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Mus musculus
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 additional information Homo sapiens PORCN splice variants differ globally in signaling activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.250 Homo sapiens Q9H237 gene PORCN
-
2.3.1.250 Mus musculus Q9JJJ7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.250 embryo
-
 Mus musculus
-
2.3.1.250 fibroblast
-
 Mus musculus
-
2.3.1.250 fibrosarcoma cell
-
 Homo sapiens
-
2.3.1.250 HT-1080 cell
-
 Homo sapiens
-
2.3.1.250 MEF cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Mus musculus CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 additional information PORCN splice variants differ globally in signaling activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.250 PORCN
-
 Homo sapiens
2.3.1.250 PORCN
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.250 malfunction gene deletion causes embryonic lethality in mice. PORCN null cells cannot activate WNT3A, PORCN null cells do not secrete WNT3A Mus musculus
2.3.1.250 malfunction mutations in gene PORCN are associated with focal dermal hypoplasia. PORCN null cells are completely incapable of autocrine Wnt signaling Homo sapiens
2.3.1.250 additional information structure-function analysis, overview Homo sapiens
2.3.1.250 additional information structure-function analysis, overview Mus musculus
2.3.1.250 physiological function the enzyme is a membrane-bound O-acyltransferase and is a key non-redundant node for the regulation of global Wnt signaling, essential regulatory role of PORCN in shaping Wnt signaling gradients. All Wnt activity absolutely requires posttranslational modification by the O-acyltransferase PORCN, whose abundance or activity modulates signaling tightly and over a large dynamic range, the enzyme activity is a key modulator of all Wnt ligand activity Homo sapiens
2.3.1.250 physiological function the enzyme is a membrane-bound O-acyltransferase and is a key non-redundant node for the regulation of global Wnt signaling, essential regulatory role of PORCN in shaping Wnt signaling gradients. All Wnt activity absolutely requires posttranslational modification by the O-acyltransferase PORCN, whose abundance or activity modulates signaling tightly and over a large dynamic range, the enzyme activity is a key modulator of all Wnt ligand activity Mus musculus