EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.131 | expression in Escherichia coli | Methanocaldococcus jannaschii |
2.7.4.31 | expression in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.31 | EDTA | 10 mM, complete loss of activity | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.31 | Mg2+ | MfnE exclusively uses Mg2+ as a cofactor to facilitate phosphoryl group transfer | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.131 | 37000 | - |
x * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
2.7.4.31 | 24000 | - |
x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.131 | Methanocaldococcus jannaschii | Q58250 | - |
- |
2.5.1.131 | Methanocaldococcus jannaschii DSM 2661 | Q58250 | - |
- |
2.6.1.108 | Methanocaldococcus jannaschii | - |
gene mfnC | - |
2.7.4.31 | Methanocaldococcus jannaschii | Q57900 | - |
- |
2.7.4.31 | Methanocaldococcus jannaschii DSM 2661 | Q57900 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.4.31 | 0.072 | - |
substrate AMP, pH 7.0, 80°C | Methanocaldococcus jannaschii |
2.7.4.31 | 0.16 | - |
substrate 5-(aminomethyl)-3-furanmethanol-phosphate, pH 7.0, 80°C | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.131 | additional information | no substrate: tyramine | Methanocaldococcus jannaschii | ? | - |
? | |
2.5.1.131 | additional information | no substrate: tyramine | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
2.5.1.131 | [5-(aminomethyl)furan-3-yl]methyl phosphate + gamma-L-glutamyltyramine | - |
Methanocaldococcus jannaschii | (4-[4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl]furan-2-yl)methanamine + diphosphate | - |
? | |
2.5.1.131 | [5-(aminomethyl)furan-3-yl]methyl phosphate + gamma-L-glutamyltyramine | - |
Methanocaldococcus jannaschii DSM 2661 | (4-[4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl]furan-2-yl)methanamine + diphosphate | - |
? | |
2.6.1.108 | L-alanine + 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate | - |
Methanocaldococcus jannaschii | pyruvate + 5-(aminomethyl)-3-furanmethanol-phosphate | - |
? | |
2.7.4.31 | AMP + ATP | - |
Methanocaldococcus jannaschii | 2 ADP | - |
? | |
2.7.4.31 | AMP + ATP | reaction of adenylate kinase, EC 2.7.4.3 | Methanocaldococcus jannaschii | 2 ADP | - |
? | |
2.7.4.31 | AMP + ATP | - |
Methanocaldococcus jannaschii DSM 2661 | 2 ADP | - |
? | |
2.7.4.31 | AMP + ATP | reaction of adenylate kinase, EC 2.7.4.3 | Methanocaldococcus jannaschii DSM 2661 | 2 ADP | - |
? | |
2.7.4.31 | ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate | - |
Methanocaldococcus jannaschii | ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate | - |
? | |
2.7.4.31 | ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate | - |
Methanocaldococcus jannaschii DSM 2661 | ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.131 | ? | x * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
2.7.4.31 | ? | x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.131 | MJ0840 | - |
Methanocaldococcus jannaschii |
2.6.1.108 | mfnC | - |
Methanocaldococcus jannaschii |
2.7.4.31 | MJ0458 | - |
Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.31 | 7 | - |
- |
Methanocaldococcus jannaschii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.131 | physiological function | enzyme catalyzes the last step in the biosynthesis of methanofuran core structure , i.e. the condensation of 5-(aminomethyl)-3-furanmethanol-diphosphate with gamma-glutamyltyramine, respectively, to form 4-[N-(gamma-L-glutamyl)-p-(beta-aminoethyl)phenoxymethyl]-(aminomethyl)furan. Condensation of the tyramine moiety with 5-(aminomethyl)-3-furanmethanol-diphosphate occurs after formation of gamma-glutamyltyramine | Methanocaldococcus jannaschii |
2.6.1.108 | evolution | all methanofuran structural variants contain a basic core structure of 4-[N-(gamma-L-glutamyl)-p-(bet-aminoethyl)phenoxymethyl]-(aminomethyl)furan (APMF-Glu) but have different side chains depending on the source organism | Methanocaldococcus jannaschii |
2.6.1.108 | metabolism | the four genes mfnA, mfnB, mfnC, and mfnD are responsible for the biosynthesis of the methanofuran precursor gamma-glutamyltyramine and 5-(aminomethyl)-3-furanmethanol-phosphate from tyrosine, glutamate, glyceraldehyde-3-phosphate, and alanine in Methanocaldococcus jannaschii, overview | Methanocaldococcus jannaschii |
2.7.4.31 | physiological function | enzyme catalyzes the formation of methanofuran precursor 5-(aminomethyl)-3-furanmethanol-diphosphate (F1-PP) from ATP | Methanocaldococcus jannaschii |