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Literature summary extracted from

  • Miggiano, R.; Casazza, V.; Garavaglia, S.; Ciaramella, M.; Perugino, G.; Rizzi, M.; Rossi, F.
    Biochemical and structural studies of the Mycobacterium tuberculosis O6-methylguanine methyltransferase and mutated variants (2013), J. Bacteriol., 195, 2728-2736.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.63 sitting drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), and 6% (w/v) polyethylene glycol 8000 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.63 R37L the mutant exhibits a 10fold-lower affinity for double stranded methylated DNA compared to the wild type enzyme Mycobacterium tuberculosis
2.1.1.63 T15S the mutant exhibits a 2fold-lower affinity for double stranded methylated DNA compared to the wild type enzyme Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.63 17844
-
 x * 17844, calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.63 DNA (containing 6-O-methylguanine) + protein L-cysteine Mycobacterium tuberculosis
-
 DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Mycobacterium tuberculosis P9WJW5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63 HiTrapQ column chromatography, MonoQ column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA (containing 6-O-methylguanine) + protein L-cysteine
-
 Mycobacterium tuberculosis DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.63 ? x * 17844, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.63 O6-methylguanine methyltransferase
-
 Mycobacterium tuberculosis
2.1.1.63 OGT
-
 Mycobacterium tuberculosis