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Literature summary extracted from

  • Strasser de Saad, A.M.; Pesce de Ruiz Holgado, A.A.; Oliver, G.
    Purification and properties of malolactic enzyme from Lactobacillus murinus CNRZ 313 (1985), J. Appl. Biochem., 6, 374-383.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.101 additional information
-
 additional information at pH values substantially different from optimum pH 5.5, a positive cooperativity between substrate molecules is observed Ligilactobacillus murinus
4.1.1.101 0.023
-
 (S)-malate 37°C, pH 5.5 Ligilactobacillus murinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.101 Mn2+ required, Km value 0.00014 mM Ligilactobacillus murinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.101 110000
-
 2 * 110000, SDS-PAGE Ligilactobacillus murinus
4.1.1.101 220000
-
 gel filtration Ligilactobacillus murinus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.101 Ligilactobacillus murinus
-
-
-
4.1.1.101 Ligilactobacillus murinus CNRZ 313
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.101
-
 Ligilactobacillus murinus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.1.1.101 presence of NAD+ protects against inactivation. Additiion of NAD+, after dissociation, restores malolactic activity Ligilactobacillus murinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.101 (S)-malate
-
 Ligilactobacillus murinus (S)-lactate + CO2
-
 ?
4.1.1.101 (S)-malate
-
 Ligilactobacillus murinus CNRZ 313 (S)-lactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.101 dimer 2 * 110000, SDS-PAGE Ligilactobacillus murinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.101 37
-
-
 Ligilactobacillus murinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.101 5.5
-
 phosphate buffer Ligilactobacillus murinus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.101 NAD+ required, Km value 0.00045 mM Ligilactobacillus murinus