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Literature summary extracted from

  • Hoshino, Y.;, Fujii, S., Shinonaga, H.; Arai, K.; Saito, F.; Fukai, T.; Satoh, H.; Miyazaki, Y.; Ishikawa, J.
    Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance (2010), J. Antibiot., 63, 23-28.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.211 gene rox, cloning and expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3) Nocardia farcinica

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.211 additional information the rox gene deletion mutation DELTArox gives no significant influence to the rifampicin resistance of Nocardia farcinica. Transformation with plasmid pKNL027_N05 overexpressing the rox gene markedly raises the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. Rifampicin is degraded by the wild-type strain, whereas it remains intact when incubated with the DELTArox strain Nocardia farcinica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.211 rifampicin + NAD(P)H + O2 Nocardia farcinica
-
 2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
 ?
1.14.13.211 rifampicin + NAD(P)H + O2 Nocardia farcinica IFM 10152
-
 2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.211 Nocardia farcinica
-
 gene rox
-
1.14.13.211 Nocardia farcinica IFM 10152
-
 gene rox
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.211 rifampicin + NAD(P)H + O2
-
 Nocardia farcinica 2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
 ?
1.14.13.211 rifampicin + NAD(P)H + O2
-
 Nocardia farcinica IFM 10152 2'-N-hydroxyrifampicin + NAD(P)+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.211 rifampicin monooxygenase
-
 Nocardia farcinica
1.14.13.211 rox
-
 Nocardia farcinica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.211 27
-
 assay at Nocardia farcinica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.211 7
-
 assay at Nocardia farcinica

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.211 FAD enzyme Rox is a probable FAD-dependent monooxygenase because it contains the FAD-binding domain (Pfam accession number PF01494) Nocardia farcinica
1.14.13.211 NAD(P)H
-
 Nocardia farcinica

General Information

EC Number General Information Comment Organism
1.14.13.211 physiological function the rox gene is capable of initiating rifampicin degradation with another metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in Nocardia farcinica Nocardia farcinica