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Literature summary extracted from

  • Zhou, Y.; Lin, X.W.; Zhang, Y.R.; Huang, Y.J.; Zhang, C.H.; Yang, Q.; Li, H.Y.; Yuan, J.Q.; Cheng, J.A.; Xu, R.; Mao, C.; Zhu, Z.R.
    Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase (2013), Insect Mol. Biol., 22, 366-375.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 additional information infection with the rice strip virus or treatment with insecticides significantly increase LsnCer mRNA expression and its enzymatic activity in Laodelphax striatellus Laodelphax striatellus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 DNA and amino acid sequence determination and analysis, recombinant overexpression in Trichopulsia ni High Five cell membranes via baculoviral transfection, expression of GFP-tagged enzyme Laodelphax striatellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Fe2+ 20% inhibition at 5 mM Laodelphax striatellus
3.5.1.23 Zn2+ 80% inhibition at 5 mM Laodelphax striatellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 79300
-
 x * 79300, about, sequence calculation Laodelphax striatellus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Laodelphax striatellus R4N4U2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 recombinant enzyme from Trichopulsia ni High Five cell membranes Laodelphax striatellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 additional information the enzyme has broad substrate specificity with preference for ceramides with a medium acyl-chain or a mono unsaturated long acyl-chain. The enzyme prefers ceramides with unsaturated acyl-chains over ceramides with saturated acyl-chains of the same length, and it does not use phytoceramides as substrates, suggesting that LsnCer is a ceramide-specific ceramidase Laodelphax striatellus ?
-
 ?
3.5.1.23 N-arachidoyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus arachidonate + D-erythro-sphingosine
-
 ?
3.5.1.23 N-hexanoyl-D-erythro-sphingosine + H2O
-
 Laodelphax striatellus hexanoate + D-erythro-sphingosine
-
 ?
3.5.1.23 N-lauroyl-D-erythro-sphingosine + H2O best substrate Laodelphax striatellus laurate + D-erythro-sphingosine
-
 ?
3.5.1.23 N-lignoceroyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus lignocerate + D-erythro-sphingosine
-
 ?
3.5.1.23 N-nervonoyl-D-erythro-sphingosine + H2O
-
 Laodelphax striatellus nervonoate + D-erythro-sphingosine
-
 ?
3.5.1.23 N-oleoyl-D-erythro-sphingosine + H2O
-
 Laodelphax striatellus oleate + D-erythro-sphingosine
-
 ?
3.5.1.23 N-palmitoyl-D-erythro-sphingosine + H2O
-
 Laodelphax striatellus palmitate + D-erythro-sphingosine
-
 ?
3.5.1.23 N-stearoyl-D-erythro-sphingosine + H2O low activity Laodelphax striatellus stearate + D-erythro-sphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 79300, about, sequence calculation Laodelphax striatellus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 LsnCer
-
 Laodelphax striatellus
3.5.1.23 nCDase
-
 Laodelphax striatellus
3.5.1.23 neutral ceramidase
-
 Laodelphax striatellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
-
 Laodelphax striatellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 8
-
-
 Laodelphax striatellus

Expression

EC Number Organism Comment Expression
3.5.1.23 Laodelphax striatellus infection with the rice strip virus or treatment with insecticides significantly increase LsnCer mRNA expression and its enzymatic activity in Laodelphax striatellus, quantitative real time PCR enzyme expression analysis up

General Information

EC Number General Information Comment Organism
3.5.1.23 physiological function LsnCer is a bona fide neutral ceramidase that may have a role in adaption of Laodelphax striatellus to environmental stresses Laodelphax striatellus