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Literature summary extracted from

  • Sandstrom, P.; Welch, W.H.; Blomquist, G.J.; Tittiger, C.
    Functional expression of a bark beetle cytochrome P450 that hydroxylates myrcene to ipsdienol (2006), Insect Biochem. Mol. Biol., 36, 835-845.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.31 expressed in Sf9 cells Ips pini

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.31 61000
-
 x * 61000, SDS-PAGE Ips pini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.31 myrcene + NADPH + H+ + O2 Ips pini myrcene is 7-methyl-3-methylene-1,6-octadiene (4R)-(-)-ipsdienol + NADP+ + H2O 81% yield, ipsidenol is 2-methyl-6-methylene-2,7-octadien-4-ol ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.31 Ips pini Q00G65 pine engraver beetle
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.31 anterior midgut
-
 Ips pini
-
1.14.14.31 fat body
-
 Ips pini
-
1.14.14.31 posterior midgut
-
 Ips pini
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.31 additional information no activity with amitinol, E-myrcenol, linalool or geraniol Ips pini ?
-
 ?
1.14.14.31 myrcene + NADPH + H+ + O2 myrcene is 7-methyl-3-methylene-1,6-octadiene Ips pini (4R)-(-)-ipsdienol + NADP+ + H2O 81% yield, ipsidenol is 2-methyl-6-methylene-2,7-octadien-4-ol ?

Subunits

EC Number Subunits Comment Organism
1.14.14.31 ? x * 61000, SDS-PAGE Ips pini

Synonyms

EC Number Synonyms Comment Organism
1.14.14.31 CYP9T2
-
 Ips pini

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.31 NADPH
-
 Ips pini

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.31 Ips pini calculated from amino acid sequence
-
 9.5