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Literature summary extracted from

  • Miao, H.X.; Qin, Y.H.; Ye, Z.X.; Hu, G.B.
    Molecular characterization and expression analysis of ubiquitin-activating enzyme E1 gene in Citrus reticulata (2013), Gene, 513, 249-259.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.45 gene CrUBE1, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons, semi-quantitative RT-PCR and quantitative real-time PCR enzyme expression anayses, recombinant expression in Pichia pastoris strain GS115 Citrus reticulata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.45 Mg2+ required Citrus reticulata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.45 49280
-
 x * 49280, sequence calculation Citrus reticulata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine Citrus reticulata
-
 AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.45 Citrus reticulata
-
 cv. Wuzishatangju (seedless) and cv. Shatangju (seedy), single copy gene CrUBE1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.45 anther expression level of the CrUBE1 gene in anthers of cv. Shatangju is approximately 10fold higher than in anthers of cv. Wuzishatangju Citrus reticulata
-
6.2.1.45 carpel high enzyme expression level Citrus reticulata
-
6.2.1.45 filament very low expression Citrus reticulata
-
6.2.1.45 flower bud
-
 Citrus reticulata
-
6.2.1.45 leaf very low expression Citrus reticulata
-
6.2.1.45 leaf bud
-
 Citrus reticulata
-
6.2.1.45 additional information semi-quantitative and quantitative expressio analysis of the enzyme in different tissues of the two cultivars, o verview, the highest expression level of CrUBE1 is detected in pistils at 7 days after self pollination of cv.Wuzishatangju, which is approximately 5fold higher than at the starting point Citrus reticulata
-
6.2.1.45 petal very low expression Citrus reticulata
-
6.2.1.45 plant ovary
-
 Citrus reticulata
-
6.2.1.45 stigma
-
 Citrus reticulata
-
6.2.1.45 style very low expression Citrus reticulata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
-
 Citrus reticulata AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.45 ? x * 49280, sequence calculation Citrus reticulata

Synonyms

EC Number Synonyms Comment Organism
6.2.1.45 UBE1
-
 Citrus reticulata
6.2.1.45 ubiquitin-activating enzyme E1
-
 Citrus reticulata

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.45 ATP
-
 Citrus reticulata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.45 Citrus reticulata sequence calculation
-
 5.44

Expression

EC Number Organism Comment Expression
6.2.1.45 Citrus reticulata self pollination induces the enzyme in cv. Wuzishatangju up

General Information

EC Number General Information Comment Organism
6.2.1.45 evolution 5 amino acids and 8 bases are different in cDNA and DNA sequences of CrUBE1 between Wuzishatangju and Shatangju, respectively Citrus reticulata
6.2.1.45 metabolism ubiquitin-activating enzyme E1 catalyzes the first step in the ubiquitination reaction, which targets a protein for degradation via a proteasome pathway. The enzyme plays an important role in metabolic processes Citrus reticulata
6.2.1.45 additional information the enzyme has six ATP binding sites, one catalytic residue, and one E2 binding domain Citrus reticulata
6.2.1.45 physiological function UBE1s catalyze the first steps in the ubiquitin conjugation cascade and are potentially involved in compatible pollination and ubiquitinmediated S-RNase degradation Citrus reticulata