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Literature summary extracted from

  • Lim, J.M.; Vikramathithan, J.; Hwangbo, K.; Ahn, J.W.; Park, Y.I.; Choi, D.W.; Jeong, W.J.
    Threonine 286 of fatty acid desaturase 7 is essential for ?-3 fatty acid desaturation in the green microalga Chlamydomonas reinhardtii (2015), Front. Microbiol., 6, 66.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.35 gene FAD7, DNA and amino acid sequence determination and analysis, genotyping, expression of T286 enzyme mutants Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.35 additional information mutation of Thr286 may directly affect the structure of the transmembrane domain of the enzyme Chlamydomonas reinhardtii
1.14.19.35 T286C site-directed mutagenesis, omega3-fatty acid deficiency mutant Chlamydomonas reinhardtii
1.14.19.35 T286G site-directed mutagenesis, omega3-fatty acid deficiency mutant Chlamydomonas reinhardtii
1.14.19.35 T286H site-directed mutagenesis, omega3-fatty acid deficiency mutant Chlamydomonas reinhardtii
1.14.19.35 T286N naturally occuring mutation causing omega3-fatty acid deficiency in strain CC-620, that can be recovered by overexpression of the wild-type enzyme from strain CC-125 Chlamydomonas reinhardtii
1.14.19.35 T286S site-directed mutagenesis, the mutant shows partially reduced enzyme activity Chlamydomonas reinhardtii
1.14.19.35 T286Y site-directed mutagenesis, omega3-fatty acid deficiency mutant Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.35 membrane
-
 Chlamydomonas reinhardtii 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.35 Chlamydomonas reinhardtii A8HMC4 gene fad7
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.19.35 phosphoprotein the phosphorylation target site is Thr286 Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
1.14.19.35 FAD7
-
 Chlamydomonas reinhardtii
1.14.19.35 fatty acid desaturase 7
-
 Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
1.14.19.35 malfunction the omega-3 fatty acids hexadecatrienoic acid (C16:3n3), hexadecatetraenoic acid (C16:4),and alpha-linolenic acid (C18:3n3) are not detected in the CC-620 strain, phenotype and fatty acid composition of the omega-3 fatty acid deficiency in strain CC-620 due to the missense mutation detected in gene CrFAD7, complementation by the wild-type enzyme, overview Chlamydomonas reinhardtii
1.14.19.35 physiological function Thr residue located in the fourth transmembrane domain of fatty acid desaturase 7 (FAD7) that is essential for the biosynthesis of omega-3 fatty acids in Chlamydomonas reinhardtii, Thr286 is essential for the maintaining the catalytic structure of the enzyme Chlamydomonas reinhardtii