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Literature summary extracted from

  • Montpellier, L.; Siemann, S.
    Effect of pH on the catalytic function and zinc content of native and immobilized anthrax lethal factor (2013), FEBS Lett., 587, 317-321.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.83 expression in Bacillus megaterium Bacillus anthracis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.83 Zn2+ content is 1 mole per mole of protein. Zinc content is retained upon acidification up to a pH value of 5.5. At pH 5.3, release of about 40% of zinc, and below pH 4.5, sharp increase in the release of zinc Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.83 Bacillus anthracis P15917
-
-

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.83 6.8 8
-
 Bacillus anthracis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.83 4
-
 inactive Bacillus anthracis
3.4.24.83 5.8
-
 progressive further loss of activity. Biphasic loss of activity upon acidification Bacillus anthracis
3.4.24.83 6.7
-
 about 60% of maximum activity. Biphasic loss of activity upon acidification Bacillus anthracis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.83 5
-
 increase in protein aggreagation between pH 4 and pH 5 Bacillus anthracis

General Information

EC Number General Information Comment Organism
3.4.24.83 metabolism about 30% demetallation is observed at pH values close to those found in late endosomes. A substantial proportion of lethal factor molecules may not be in their zinc-bound state prior to translocation Bacillus anthracis