Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pan, M.; Santangelo, T.J.; Cubonova, L.; Li, Z.; Metangmo, H.; Ladner, J.; Hurwitz, J.; Reeve, J.N.; Kelman, Z.
    Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability (2013), Extremophiles, 17, 453-461.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.B8 Mg2+ required Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.4.B8 37200
-
 1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE Thermococcus kodakarensis
3.6.4.B8 58100
-
 1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE Thermococcus kodakarensis
3.6.4.B8 203000
-
 light scattering Thermococcus kodakarensis
3.6.4.B8 208000
-
 gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.B8 ATP + H2O Thermococcus kodakarensis
-
 ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O Thermococcus kodakarensis KW128
-
 ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B8 Thermococcus kodakarensis
-
-
-
3.6.4.B8 Thermococcus kodakarensis KW128
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B8 ATP + H2O
-
 Thermococcus kodakarensis ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O
-
 Thermococcus kodakarensis KW128 ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.4.B8 pentamer 1 * 58100, subunit RFC-L, + 4 * 37200, subunit RFC-S, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B8 replication factor C
-
 Thermococcus kodakarensis
3.6.4.B8 RFC
-
 Thermococcus kodakarensis
3.6.4.B8 RFC complex
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.4.B8 70
-
 assay at Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.B8 8
-
 assay at Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
3.6.4.B8 evolution most archaeal genomes also encode two RFC homologs, designated the RFC large (RFC-L) and small (RFC-S) subunits, that assemble to form a pentameric complex that contains one RFC-L and four RFC-S subunits Thermococcus kodakarensis
3.6.4.B8 physiological function proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential for DNA metabolic processes. The PCNA clamp does not assemble autonomously but is loaded onto DNA by the replication factor C (RFC) clamp loader complex. RFC recognizes the 3' end of a single-strand/duplex DNA (primer-template) junction and uses the energy of ATP hydrolysis to assemble the PCNA ring around the primer. Primer extension by PolB is completely dependent on the presence of RFC plus either PCNA1 or PCNA2, and the rate of DNA synthesis increases when the PCNA1 or PCNA2 concentration is increased Thermococcus kodakarensis