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Literature summary extracted from

  • Hutton, M.; Day, J.M.; Bizouarn, T.; Jackson, J.B.
    Kinetic resolution of the reaction catalysed by proton-translocating transhydrogenase from Escherichia coli as revealed by experiments with analogues of the nucleotide substrates (1994), Eur. J. Biochem., 219, 1041-1051.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.1 expressed in Escherichia coli JM83 cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.1 N,N'-dicyclohexylcarbodiimide modification of the enzyme with N,N'-dicyclohexylcarbodiimide leads to inhibition of the rate of release of NADP+ and NADPH from the enzyme, but has a much smaller effect on the binding and release of NAD+, NADH and their analogues and on the interconversion of the ternary complexes of the enzyme with its substrates Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.1 49000
-
 1 * 54000 + 1 * 49000, SDS-PAGE Escherichia coli
7.1.1.1 54000
-
 1 * 54000 + 1 * 49000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+[side 1] Escherichia coli
-
 NADP+ + NADH + H+[side 2]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.1 phenyl-Sepharose column chromatography and DEAE-Trisacryl M column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.1 0.11
-
 with oxidized acetyl pyridine adenine dinucleotide and deamino-NADPH as substrates, at pH 6.0 and 25°C Escherichia coli
7.1.1.1 0.47
-
 with oxidized acetyl pyridine adenine dinucleotide and 1-N6-etheno-NADPH as substrates, at pH 6.0 and 25°C Escherichia coli
7.1.1.1 1.46
-
 with oxidized acetyl pyridine adenine dinucleotide and NADPH as substrates, at pH 6.0 and 25°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.1 1,N6-etheno-NADPH + NAD+ + H+[side 1]
-
 Escherichia coli 1,N6-etheno-NADP+ + NADH + H+[side 2]
-
 r
7.1.1.1 1,N6-etheno-NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
 Escherichia coli 1,N6-etheno-NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
 r
7.1.1.1 deamino-NADPH + NAD+ + H+[side 1]
-
 Escherichia coli deamino-NADP+ + NADH + H+[side 2]
-
 r
7.1.1.1 deamino-NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
 Escherichia coli deamino-NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
 r
7.1.1.1 NADPH + NAD+ + H+[side 1]
-
 Escherichia coli NADP+ + NADH + H+[side 2]
-
 r
7.1.1.1 NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
 Escherichia coli NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
 r

Subunits

EC Number Subunits Comment Organism
7.1.1.1 heterodimer 1 * 54000 + 1 * 49000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.1.1.1 H+-transhydrogenase
-
 Escherichia coli
7.1.1.1 proton-translocating transhydrogenase
-
 Escherichia coli