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Literature summary extracted from

  • Zไuner, S.; Jochum, W.; Bigorowski, T.; Benning, C.
    A Cytochrome b5-containing plastid-located fatty acid desaturase from Chlamydomonas reinhardtii (2012), Eukaryot. Cell, 11, 856-863.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.31 gene CrDELTA4FAD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GFP-tagged enzyme in Chlamydomonas reinhardtii chloroplasts, recombinant expression of the His-tagged cytochrome b5 domain (resdues 48-147) in Escherichia coli strain BL21(DE3) Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.31 chloroplast plastid envelope Chlamydomonas reinhardtii 9507
-
1.14.19.31 endoplasmic reticulum
-
 Chlamydomonas reinhardtii 5783
-
1.14.19.31 membrane membrane-bound enzyme, showing all characteristic features of a membrane-bound desaturase, including three histidine boxes and a transit peptide for chloroplast targeting Chlamydomonas reinhardtii 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.31 Chlamydomonas reinhardtii I2CYZ4 gene CrDELTA4FAD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.31 recombinant His-tagged cytochrome b5 domain (residues 48-147) from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.31 additional information the recombinant His-tagged cytochrome b5 domain of the enzyme is active in vitro Chlamydomonas reinhardtii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.31 CrDELTA4FAD
-
 Chlamydomonas reinhardtii
1.14.19.31 MGDG-specific DELTA4-desaturase
-
 Chlamydomonas reinhardtii
1.14.19.31 monogalactosyldiacylglycerol-specific DELTA4-desaturase
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.31 cytochrome b5 cytochrome b5 is the primary electron donor for endoplasmic reticulum desaturases. The soluble cytochrome b5 domain corresponds to amino acids 48 to 147 Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
1.14.19.31 malfunction enzyme knockdown lines show decreases in the 16:4 and 18:3-9,12,15 acyl groups in lipids, fatty acid profile, overview Chlamydomonas reinhardtii
1.14.19.31 additional information overproduction of the enzyme in Chlamydomonas not only increases levels of 16:4 acyl groups in cell extracts but specifically increases the total amount of monogalactosyldiacylglycerol, fatty acid profile, overview Chlamydomonas reinhardtii
1.14.19.31 physiological function the enzyme is involved in synthesis of the plastid lipid monogalactosyldiacylglycerol (MGDG) in the green alga Chlamydomonas reinhardtii occuring preferentially as a molecular species containing alpha-linolenic acid 18:3-9,12,15 in the sn-1 position and (4Z,7Z,10Z,13Z)-hexadeca-4,7,10,13-tetraenoic acid (16:4) in the sn-2 position of the glycerol backbone, fatty acid profile, overview Chlamydomonas reinhardtii