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Literature summary extracted from

  • Lee, S.; Kim, J.; La, I.; Kim, S.; Yoon, M.
    Characterization of recombinant FAD-independent catabolic acetolactate synthase from Enterococcus faecalis V583 (2013), Enzyme Microb. Technol., 52, 54-59.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.2.1.6 additional information no activation by FAD Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 expressed in Escherichia coli BL21(DE3) cells Enterococcus faecalis
2.2.1.6 gene als, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 additional information
-
 additional information Michaelis-Menten kinetics and optimal reaction conditions, overview Enterococcus faecalis
2.2.1.6 1.37
-
 pyruvate pH 6.8, 37°C, recombinant enzyme Enterococcus faecalis
2.2.1.6 1.37
-
 pyruvate at pH 6.8 and 37°C Enterococcus faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Enterococcus faecalis
2.2.1.6 Mg2+ independent Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 60000
-
 x * 60000, SDS-PAGE Enterococcus faecalis
2.2.1.6 60000
-
 x * 60000, recombinant enzyme, SDS-PAGE Enterococcus faecalis
2.2.1.6 60200
-
 x * 60200, calculated from amino acid sequence Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 2 pyruvate Enterococcus faecalis
-
 2-acetolactate + CO2
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.2.1.6 Acetone the recombinant enzyme shows a loss of over 50% activity Enterococcus faecalis
2.2.1.6 Acetone the activity decreases to less than 50% in the presence of 15% (v/v) acetone Enterococcus faecalis
2.2.1.6 DMSO the recombinant enzyme is extremely stable in DMSO Enterococcus faecalis
2.2.1.6 DMSO the enzyme exhibits extreme stability in the presence of up to 20% (v/v) DMSO Enterococcus faecalis
2.2.1.6 Ethanol the recombinant enzyme shows a loss of over 50% activity Enterococcus faecalis
2.2.1.6 Ethanol the activity decreases to less than 50% in the presence of 15% (v/v) ethanol and to 7% at 20% (v/v) ethanol Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Enterococcus faecalis Q836A5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 Ni-Sepharose column chromatography Enterococcus faecalis
2.2.1.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinit chromatography and gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Enterococcus faecalis 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 60000, SDS-PAGE Enterococcus faecalis
2.2.1.6 ? x * 60000, recombinant enzyme, SDS-PAGE Enterococcus faecalis
2.2.1.6 ? x * 60200, calculated from amino acid sequence Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 ALS
-
 Enterococcus faecalis
2.2.1.6 CalS
-
 Enterococcus faecalis
2.2.1.6 catabolic acetolactate synthase
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 37
-
-
 Enterococcus faecalis
2.2.1.6 37
-
 recombinant enzyme Enterococcus faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.6 40
-
 the activity above 40°C is less than 20% and then completely decreases at higher temperatures Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.6 5.3
-
 pyruvate at pH 6.8 and 37°C Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 6.8
-
-
 Enterococcus faecalis
2.2.1.6 6.8
-
 recombinant enzyme Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 additional information the enzyme is not FAD-dependent Enterococcus faecalis
2.2.1.6 thiamine diphosphate
-
 Enterococcus faecalis
2.2.1.6 thiamine diphosphate dependent on Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.6 3.8
-
 pyruvate at pH 6.8 and 37°C Enterococcus faecalis