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Literature summary extracted from

  • Galli, L.; Barnes, T.; Secrest, S.; Kadowaki, T.; Burrus, L.
    Porcupine-mediated lipid-modification regulates the activity and distribution of Wnt proteins in the chick neural tube (2007), Development, 134, 3339-3348.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.250 overexpression of HA-tagged human PORC to confirm the ability of hPORC RNAi constructs to knockdown hPORC expression Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.250 H341D site-directed mutagenesis, catalytically inactive mutant Mus musculus
2.3.1.250 H341Q site-directed mutagenesis, catalytically inactive mutant Mus musculus
2.3.1.250 additional information overexpression of HA-tagged human PORC to confirm the ability of hPORC RNAi constructs to knockdown hPORC expression Homo sapiens
2.3.1.250 additional information the catalytically inactive porcupine is able to act as an inhibitor of endogenous porcupine, possibly via direct competition for substrate Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine Gallus gallus
-
 CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine Homo sapiens
-
 CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine Mus musculus
-
 CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Gallus gallus
-
 CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Homo sapiens
-
 CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine Mus musculus
-
 CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.250 Gallus gallus
-
-
-
2.3.1.250 Homo sapiens Q9H237
-
-
2.3.1.250 Mus musculus Q9JJJ7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.250 HEK-293T cell
-
 Homo sapiens
-
2.3.1.250 neural tube
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
-
 Gallus gallus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
-
 Homo sapiens CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
-
 Mus musculus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-) Gallus gallus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-) Homo sapiens CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-) Mus musculus CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
-
 Gallus gallus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
-
 Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
-
 Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine chicken substrate, and substrate from Mus musculus Gallus gallus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine chicken substrate, and substrate from Mus musculus Homo sapiens CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine chicken substrate, and substrate from Mus musculus Mus musculus CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 additional information vertebrate Wnt1 and Wnt3a possess at least one additional site for porcupine-mediated lipid-modification Homo sapiens ?
-
 ?
2.3.1.250 additional information vertebrate Wnt1 and Wnt3a possess at least one additional site for porcupine-mediated lipid-modification, localization of Wnt proteins in the chick neural tube, overview Gallus gallus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.250 mPorcD
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.250 malfunction the catalytically inactive porcupine is able to act as an inhibitor of endogenous porcupine, possibly via direct competition for substrate Mus musculus
2.3.1.250 physiological function porcupine-mediated lipid-modification regulates the activity and distribution of Wnt proteins, and promotes their activity in 293T cells, porcupine is required for Wnt activity in 293T cells, and porcupine/palmitoylation regulates the activity of Wnt1 and Wnt3a in 293T cells Homo sapiens
2.3.1.250 physiological function porcupine-mediated lipid-modification regulates the activity and distribution of Wnt proteins, and promotes their activity in the chick neural tube, while it reduces the range of activity of Wnt1 and Wnt3a in the chick neural tube Mus musculus
2.3.1.250 physiological function porcupine-mediated lipid-modification regulates the activity and distribution of Wnt proteins, it promotes the activity of endogenous Wnt1 and Wnt3a in the chick neural tube, ectopic expression of mouse porcupine in the chick neural tube steepens the Wnt1/Wnt3a proliferation gradient, but porcupine expression restricts the Wnt activity range and distribution in the chicken neural tube, overview Gallus gallus