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Literature summary extracted from

  • Yang, S.; Zhang, F.; Kang, J.; Zhang, W.; Deng, G.; Xin, Y.; Ma, Y.
    Mycobacterium tuberculosis Rv1096 protein: gene cloning, protein expression, and peptidoglycan deacetylase activity (2014), BMC Microbiol., 14, 174.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.104 gene Rv1096, sequence comparisons, recombinant expression in Escherichia coli strain ER2566 and in Mycobacterium smegmatis strain mc2155, deacetylation of cell wall peptidoglycan by the recombinant enzyme leads to lysozyme resistance in Mycobacterium smegmatis Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.104 EDTA
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.104 0.91
-
 peptidoglycan-N-acetyl-D-glucosamine recombinant enzyme, pH 7.0, 37°C Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.104 cell wall
-
 Mycobacterium tuberculosis 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.104 Co2+ activates, best metal ion Mycobacterium tuberculosis
3.5.1.104 additional information metallo-dependent enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O Mycobacterium tuberculosis
-
 peptidoglycan-D-glucosamine + acetate
-
 ?
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O Mycobacterium tuberculosis H37Rv
-
 peptidoglycan-D-glucosamine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.104 Mycobacterium tuberculosis O53444 gene Rv1096
-
3.5.1.104 Mycobacterium tuberculosis H37Rv O53444 gene Rv1096
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Mycobacterium tuberculosis peptidoglycan-D-glucosamine + acetate
-
 ?
3.5.1.104 peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Mycobacterium tuberculosis H37Rv peptidoglycan-D-glucosamine + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.104 Rv1096 protein
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.104 37
-
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.104 0.099
-
 peptidoglycan-N-acetyl-D-glucosamine recombinant enzyme, pH 7.0, 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.104 7
-
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.5.1.104 physiological function many bacteria modulate and evade the immune defenses of their hosts through peptidoglycan deacetylation. Deacetylation of cell wall peptidoglycan by the recombinant enzyme leads to lysozyme resistance in Mycobacterium smegmatis Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.104 1.088
-
 peptidoglycan-N-acetyl-D-glucosamine recombinant enzyme, pH 7.0, 37°C Mycobacterium tuberculosis