BRENDA - Enzyme Database

Photosystem I from plants as a bacterial cytochrome P450 surrogate electron donor: terminal hydroxylation of branched hydrocarbon chains

Jensen, K.; Johnston, J.B.; de Montellano, P.R.; M?ller, B.L.; Biotechnol. Lett. 34, 239-245 (2012)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.14
0.021
-
farnesol
at pH 7.5 and 25C
Mycobacterium tuberculosis
1.14.15.14
0.036
-
cholest-4-en-3-one
at pH 7.5 and 25C
Mycobacterium tuberculosis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.14
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
Mycobacterium tuberculosis
the enzyme uses spinach NAD(P)H-dependent ferredoxin reductase and the [2Fe-2S] iron-sulfur protein ferredoxin as an electron transport system
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.14
Mycobacterium tuberculosis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.14
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
the enzyme uses spinach NAD(P)H-dependent ferredoxin reductase and the [2Fe-2S] iron-sulfur protein ferredoxin as an electron transport system
733555
Mycobacterium tuberculosis
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
cholest-4-en-3-one + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
733555
Mycobacterium tuberculosis
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
733555
Mycobacterium tuberculosis
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
733555
Mycobacterium tuberculosis
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
additional information
the enzyme shows very low activity towards palmitic acid and 15-methyl-palmitic acid
733555
Mycobacterium tuberculosis
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.15.14
0.195
-
cholest-4-en-3-one
at pH 7.5 and 25C
Mycobacterium tuberculosis
1.14.15.14
0.258
-
farnesol
at pH 7.5 and 25C
Mycobacterium tuberculosis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.14
NADPH
-
Mycobacterium tuberculosis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.14
NADPH
-
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.14
0.021
-
farnesol
at pH 7.5 and 25C
Mycobacterium tuberculosis
1.14.15.14
0.036
-
cholest-4-en-3-one
at pH 7.5 and 25C
Mycobacterium tuberculosis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.14
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
Mycobacterium tuberculosis
the enzyme uses spinach NAD(P)H-dependent ferredoxin reductase and the [2Fe-2S] iron-sulfur protein ferredoxin as an electron transport system
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.14
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
the enzyme uses spinach NAD(P)H-dependent ferredoxin reductase and the [2Fe-2S] iron-sulfur protein ferredoxin as an electron transport system
733555
Mycobacterium tuberculosis
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
cholest-4-en-3-one + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
733555
Mycobacterium tuberculosis
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
733555
Mycobacterium tuberculosis
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
733555
Mycobacterium tuberculosis
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
1.14.15.14
additional information
the enzyme shows very low activity towards palmitic acid and 15-methyl-palmitic acid
733555
Mycobacterium tuberculosis
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.15.14
0.195
-
cholest-4-en-3-one
at pH 7.5 and 25C
Mycobacterium tuberculosis
1.14.15.14
0.258
-
farnesol
at pH 7.5 and 25C
Mycobacterium tuberculosis