Literature summary extracted from
Saitou, H.; Watanabe, M.; Maruyama, K.
Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3 (2012), Biosci. Biotechnol. Biochem., 76, 1239-1241.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.7.1.24 |
additional information |
no effect on enzyme activity by 2,2-dipyridine, 8-hydroxyquinoline, 1,1-phenanthroline, tiron, and EDTA |
Pseudomonas sp. |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.7.1.24 |
gene phlG, from locus phlHGFACBDEI, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain XL-1 Blue |
Pseudomonas sp. |
General Stability
EC Number |
General Stability |
Organism |
---|
3.7.1.24 |
2-mercaptoethanol or DTT is required for stability, the purified enzyme is rapidly inactivates without stabilizing compounds, the enzyme can be partially reactivated by 10 mM 2-mercaptoethanol to regain 20% of its initial activity, the enzyme is irreversibly inactivated by freezing and thawing |
Pseudomonas sp. |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.7.1.24 |
Cu2+ |
slight inhibition |
Pseudomonas sp. |
|
3.7.1.24 |
diethyldicarbonate |
60% inhibition at 0.5 mM |
Pseudomonas sp. |
|
3.7.1.24 |
monoacetylphloroglucinol |
competitive inhibition |
Pseudomonas sp. |
|
3.7.1.24 |
additional information |
no inhibition by 2-hydroxyacetophenone and 4-hydroxyacetophenone. No effect on enzyme activity by 2,2-dipyridine, 8-hydroxyquinoline, 1,1-phenanthroline, tiron, and EDTA |
Pseudomonas sp. |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.7.1.24 |
0.024 |
- |
2,4-diacetylphloroglucinol |
recombinant enzyme, pH 7.0, 25°C |
Pseudomonas sp. |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.7.1.24 |
Co2+ |
specifically and significantly activates the enzyme, shows a hyperbolic saturation curve in the micromolar range, binding to the enzyme might induce a conformational change that increases the catalytic effect of Zn2+ or might forma a distinct catalytic site besides that for Zn2+ |
Pseudomonas sp. |
|
3.7.1.24 |
additional information |
the enzyme has a 168HXHXD172 metal binding motif. Poor effects on enzyme activity by Ca2+, Ni2+, Fe3+, Zn2+, and Mg2+ |
Pseudomonas sp. |
|
3.7.1.24 |
Zn2+ |
a zinc hydrolase, located at the bottom of a deep narrow pocket of the enzyme molecule, coordinated by H129, E160, H270, and E274 |
Pseudomonas sp. |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.7.1.24 |
35000 |
- |
2 * 35000, recombinant enzyme, SDS-PAGE |
Pseudomonas sp. |
3.7.1.24 |
65000 |
- |
recombinant enzyme, gel filtration |
Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.7.1.24 |
2,4-diacetylphloroglucinol + H2O |
Pseudomonas sp. |
- |
2-acetylphloroglucinol + acetate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.7.1.24 |
Pseudomonas sp. |
F7J5X9 |
gene phlG |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.7.1.24 |
recombinant enzyme 22fold from Escherichia coli strain XL-1 Blue to homogeneity by ammonium sulfate fractionation, gel filtration, two steps of anion exchange chromatography, and hydrophobic interaction chromatograpy |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.7.1.24 |
9.1 |
- |
purified recombinant enzyme, pH 7.0, 25°C |
Pseudomonas sp. |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.7.1.24 |
2,4-diacetylphloroglucinol + H2O |
- |
Pseudomonas sp. |
2-acetylphloroglucinol + acetate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.7.1.24 |
homodimer |
2 * 35000, recombinant enzyme, SDS-PAGE |
Pseudomonas sp. |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.7.1.24 |
Phlg |
- |
Pseudomonas sp. |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.7.1.24 |
25 |
- |
assay at |
Pseudomonas sp. |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.7.1.24 |
47 |
55 |
purified recombinant enzyme, 50% activity remaining at 47°C, inactivation at 55°C |
Pseudomonas sp. |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.7.1.24 |
5.8 |
- |
2,4-diacetylphloroglucinol |
recombinant enzyme, pH 7.0, 25°C |
Pseudomonas sp. |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.7.1.24 |
6 |
- |
- |
Pseudomonas sp. |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
3.7.1.24 |
0.087 |
- |
monoacetylphloroglucinol |
recombinant enzyme, pH 7.0, 25°C |
Pseudomonas sp. |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.7.1.24 |
physiological function |
2,4-diacetylphloroglucinol is produced by Pseudomonas fluorescens an antibiotic that represses the growth of pathogenic microorganisms in the rhizosphere of host plants, the diacetylphloroglucinol hydrolase is a key compound in the biocontrol of Pseudomonas fluorescens |
Pseudomonas sp. |