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Literature summary extracted from

  • Maier, J.; Albu, R.; Jurkowski, T.; Jeltsch, A.
    Investigation of the C-terminal domain of the bacterial DNA-(adenine N6)-methyltransferase CcrM (2015), Biochimie, 119, 60-67.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 D347A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 H317A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
2.1.1.72 H336A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
2.1.1.72 K318A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 N330A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
2.1.1.72 Q324A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 R272A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
2.1.1.72 R302A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
2.1.1.72 R350A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
2.1.1.72 R354A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 S315A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.72 39000
-
 x * 39000, SDS-PAGE Caulobacter vibrioides

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Caulobacter vibrioides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 Ni-NTA agarose column chromatography Caulobacter vibrioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information the enzyme methylates GANTC sequences but does not specifically bind to DNA containing GANTC sequences Caulobacter vibrioides ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine a biotinylated double stranded 23mer oligodeoxynucleotide with one hemimethylated GANTC site is used as substrate Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 ? x * 39000, SDS-PAGE Caulobacter vibrioides

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 CcrM
-
 Caulobacter vibrioides
2.1.1.72 DNA-(adenine N6)-methyltransferase
-
 Caulobacter vibrioides