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Literature summary extracted from

  • Elsawy, H.; Chahar, S.
    Increasing DNA substrate specificity of the EcoDam DNA-(adenine N6)-methyltransferase by site-directed mutagenesis (2014), Biochemistry, 79, 1262-1266.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli HMS174 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 L122A the mutant is almost inactive on unmethylated DNA. The variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA with 35-40% of wild type activity Escherichia coli
2.1.1.72 L122A/P134A the variant methylates hemimethylated DNA with 80% of wild type activity Escherichia coli
2.1.1.72 L122A/V133L the mutant shows increased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli
2.1.1.72 L122A/V133L the variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA Escherichia coli
2.1.1.72 L122G the mutant has no preference for hemimethylated substrate and is about 1.5fold more active than the wild type enzyme on the hemimethylated DNA substrate Escherichia coli
2.1.1.72 L122I the variant methylates hemimethylated DNA with 98% of wild type activity Escherichia coli
2.1.1.72 L122S the variant is able to sense the methylation status of the 5'-GATC-3' double-stranded target recognition site and methylates only hemimethylated DNA with 35-40% of wild type activity Escherichia coli
2.1.1.72 L122T the variant methylates hemimethylated DNA with 90% of wild type activity Escherichia coli
2.1.1.72 L122V the variant methylates hemimethylated DNA with 95% of wild type activity Escherichia coli
2.1.1.72 P134A the variant methylates hemimethylated DNA with 100% of wild type activity Escherichia coli
2.1.1.72 V133A the mutant shows increased activity with hemimethylated DNA compared to the wild type enzyme (160%) Escherichia coli
2.1.1.72 V133I the mutant shows decreased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli
2.1.1.72 V133L the mutant shows most strongly decreased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli
2.1.1.72 V133S the mutant shows strongly decreased activity with hemimethylated DNA compared to the wild type enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.72 30000
-
 x * 30000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 additional information Escherichia coli the wild type enzyme is active on hemimethylated and unmethylated DNA substrates ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli
-
 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli P0AEE8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 Ni-NTA column chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information the wild type enzyme is active on hemimethylated and unmethylated DNA substrates Escherichia coli ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine
-
 Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 ? x * 30000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 Dam
-
 Escherichia coli
2.1.1.72 DNA-(adenine N6)-methyltransferase
-
 Escherichia coli