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Literature summary extracted from

  • Kubiak, R.L.; Thoden, J.B.; Holden, H.M.
    Structure of EvaA: a paradigm for sugar 2,3-dehydratases (2013), Biochemistry, 52, 2078-2088.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.159
-
 Amycolatopsis orientalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.159 to 1.7 A resolution. Each subunit of the EvaA dimer folds into two domains that clearly arose via gene duplication. Two dTDP-sugar binding pockets, A and B, are present in each EvaA subunit. Pocket A functions as the active site and pocket B is simply a remnant left behind from the gene duplication event Amycolatopsis orientalis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.159 E139Q complete loss of catalytic activity Amycolatopsis orientalis
4.2.1.159 E190A complete loss of catalytic activity Amycolatopsis orientalis
4.2.1.159 E190Q complete loss of catalytic activity Amycolatopsis orientalis
4.2.1.159 E231A/E233A mutant constructed to reduce surface entropy. None of the resulting crystals demonstrates improved diffraction properties Amycolatopsis orientalis
4.2.1.159 E316A/K317A mutant constructed to reduce surface entropy. None of the resulting crystals demonstrates improved diffraction properties Amycolatopsis orientalis
4.2.1.159 E405 complete loss of catalytic activity Amycolatopsis orientalis
4.2.1.159 E405A complete loss of catalytic activity Amycolatopsis orientalis
4.2.1.159 K289A/Q290A/E293A mutant constructed to reduce surface entropy. None of the resulting crystals demonstrates improved diffraction properties Amycolatopsis orientalis
4.2.1.159 R381A mutant with improved crystallization parameters, kinetic parameters similar to wild-type Amycolatopsis orientalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.159 0.038
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose mutant R381A, pH 7.5, 25°C Amycolatopsis orientalis
4.2.1.159 0.056
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose wild-type, pH 7.5, 25°C Amycolatopsis orientalis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.159 Amycolatopsis orientalis O52793
-
-
4.2.1.159 Amycolatopsis orientalis NRRL18098 O52793
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.159 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
-
 Amycolatopsis orientalis dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + H2O overall reaction ?
4.2.1.159 dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
-
 Amycolatopsis orientalis NRRL18098 dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose + H2O overall reaction ?

Subunits

EC Number Subunits Comment Organism
4.2.1.159 dimer crystallization data Amycolatopsis orientalis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.159 evaA
-
 Amycolatopsis orientalis