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Literature summary extracted from

  • Steinborn, G.; Hajirezaei, M.R.; Hofemeister, J.
    bac genes for recombinant bacilysin and anticapsin production in Bacillus host strains (2005), Arch. Microbiol., 183, 71-79.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.49 gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322 Bacillus subtilis
6.3.2.49 gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322 Bacillus amyloliquefaciens
6.3.2.49 gene bacD, genetic structure of gene cluster bacABCDE, complementation study with bacD genes from several other Bacillus sources, overview Bacillus subtilis
6.3.2.49 gene bacD, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322 Bacillus pumilus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.49 additional information several bacilysin-deficient strains, e.g. strain ng79, construction of mutant strains GSB315, GSB319, GSB320, GSB321, and GSB322 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.49 Mg2+
-
 Bacillus pumilus
6.3.2.49 Mg2+
-
 Bacillus subtilis
6.3.2.49 Mg2+
-
 Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.49 ATP + L-alanine + L-anticapsin Bacillus pumilus
-
 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin Bacillus subtilis
-
 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin Bacillus amyloliquefaciens
-
 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin Bacillus subtilis 168
-
 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin Bacillus pumilus ATCC 7065
-
 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin Bacillus amyloliquefaciens ATCC 15841
-
 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin Bacillus subtilis A1/3
-
 ADP + phosphate + bacilysin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.49 Bacillus amyloliquefaciens Q8KWS8 with plasmid pSB657, gene bacD
-
6.3.2.49 Bacillus amyloliquefaciens ATCC 15841 Q8KWS8 with plasmid pSB657, gene bacD
-
6.3.2.49 Bacillus pumilus
-
 gene bacD
-
6.3.2.49 Bacillus pumilus ATCC 7065
-
 gene bacD
-
6.3.2.49 Bacillus subtilis P39641 with plasmid pSB657, gene bacD or ywfE, gene cluster ywfBCDEF or bacABCDE
-
6.3.2.49 Bacillus subtilis Q8KWT3 with plasmid pSB657, gene bacD
-
6.3.2.49 Bacillus subtilis 168 P39641 with plasmid pSB657, gene bacD or ywfE, gene cluster ywfBCDEF or bacABCDE
-
6.3.2.49 Bacillus subtilis A1/3 Q8KWT3 with plasmid pSB657, gene bacD
-
6.3.2.49 no activity in Bacillus amyloliquefaciens
-
-
-
6.3.2.49 no activity in Bacillus amyloliquefaciens GSB272
-
-
-
6.3.2.49 no activity in Bacillus coagulans
-
-
-
6.3.2.49 no activity in Bacillus licheniformis
-
-
-
6.3.2.49 no activity in Bacillus licheniformis ATCC 9789
-
-
-
6.3.2.49 no activity in Bacillus megaterium
-
-
-
6.3.2.49 no activity in Bacillus megaterium PV361
-
-
-
6.3.2.49 no activity in Bacillus pumilus
-
-
-
6.3.2.49 no activity in Bacillus pumilus ATCC 12140
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.49 ATP + L-alanine + L-anticapsin
-
 Bacillus pumilus ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin
-
 Bacillus subtilis ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin
-
 Bacillus amyloliquefaciens ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin
-
 Bacillus subtilis 168 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin
-
 Bacillus pumilus ATCC 7065 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin
-
 Bacillus amyloliquefaciens ATCC 15841 ADP + phosphate + bacilysin
-
 ?
6.3.2.49 ATP + L-alanine + L-anticapsin
-
 Bacillus subtilis A1/3 ADP + phosphate + bacilysin
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.49 ATP
-
 Bacillus pumilus
6.3.2.49 ATP
-
 Bacillus subtilis
6.3.2.49 ATP
-
 Bacillus amyloliquefaciens

General Information

EC Number General Information Comment Organism
6.3.2.49 malfunction mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant Bacillus subtilis
6.3.2.49 malfunction mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant Bacillus amyloliquefaciens
6.3.2.49 additional information physiological prevention of suicidal antibiotic effects, e.g. for enzyme-deficient strain GSB322 with plasmid pSB674(bacABCD), overview Bacillus subtilis
6.3.2.49 physiological function the enzyme shows antibiotic activity against various indicator strains as Candida albicans CAP1-312, Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae Bacillus subtilis
6.3.2.49 physiological function the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae Bacillus subtilis
6.3.2.49 physiological function the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae Bacillus amyloliquefaciens
6.3.2.49 physiological function the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae Bacillus pumilus