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Literature summary extracted from

  • Zummo, F.P.; Marineo, S.; Pace, A.; Civiletti, F.; Giardina, A.; Puglia, A.M.
    Tryptophan catabolism via kynurenine production in Streptomyces coelicolor: identification of three genes coding for the enzymes of tryptophan to anthranilate pathway (2012), Appl. Microbiol. Biotechnol., 94, 719-728.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.9 gene kynB or SCO3644, gene expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.9 0.23
-
 N-formyl-L-kynurenine pH 7.4, temperature not specified in the publication, recombinant His6-tagged enzyme Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.9 Zn2+ required Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.9 N-formyl-L-kynurenine + H2O Streptomyces coelicolor
-
 formate + L-kynurenine
-
 ?
3.5.1.9 N-formyl-L-kynurenine + H2O Streptomyces coelicolor M145
-
 formate + L-kynurenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.9 Streptomyces coelicolor Q9X8R1 gene SCO3644; gene kynB or SCO3644
-
3.5.1.9 Streptomyces coelicolor M145 Q9X8R1 gene SCO3644; gene kynB or SCO3644
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.9 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.9 3
-
 pH 7.4, temperature not specified in the publication, purified recombinant His6-tagged enzyme Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.9 N-formyl-L-kynurenine + H2O
-
 Streptomyces coelicolor formate + L-kynurenine
-
 ?
3.5.1.9 N-formyl-L-kynurenine + H2O
-
 Streptomyces coelicolor M145 formate + L-kynurenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.9 KFA
-
 Streptomyces coelicolor
3.5.1.9 kynurenine formamidase
-
 Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.9 7.4
-
 assay at Streptomyces coelicolor

Expression

EC Number Organism Comment Expression
3.5.1.9 Streptomyces coelicolor tryptophan positively affects kynB transcription up

General Information

EC Number General Information Comment Organism
3.5.1.9 metabolism the enzyme is involved in the tryptophan-to-anthranilate pathway Streptomyces coelicolor