BRENDA - Enzyme Database

Mutation study of conserved amino acid residues of Spirulina DELTA6-acyl-lipid desaturase showing involvement of histidine 313 in the regioselectivity of the enzyme

Hongsthong, A.; Subudhi, S.; Sirijuntarat, M.; Cheevadhanarak, S.; Appl. Microbiol. Biotechnol. 66, 74-84 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.46
expressed in Escherichia coli DH5alpha cells
Arthrospira platensis
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.46
D138N
inactive
Arthrospira platensis
1.14.19.46
E140Q
inactive
Arthrospira platensis
1.14.19.46
G136H
inactive
Arthrospira platensis
1.14.19.46
H124R
inactive
Arthrospira platensis
1.14.19.46
H128R
inactive
Arthrospira platensis
1.14.19.46
H129R
inactive
Arthrospira platensis
1.14.19.46
H305R
the mutant shows 17% of wild type activity
Arthrospira platensis
1.14.19.46
H306R
inactive
Arthrospira platensis
1.14.19.46
H313R
inactive
Arthrospira platensis
1.14.19.46
H315N
inactive
Arthrospira platensis
1.14.19.46
H89R
inactive
Arthrospira platensis
1.14.19.46
H93R
the mutant shows 11% of wild type activity
Arthrospira platensis
1.14.19.46
R123N
the mutant shows 91% of wild type activity
Arthrospira platensis
1.14.19.46
W294G
inactive
Arthrospira platensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.19.46
0.5
-
linoleic acid
mutant enzyme R123N, at pH 7.5 and 25°C; wild type enzyme, at pH 7.5 and 25°C
Arthrospira platensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.46
47000
-
x * 47000, SDS-PAGE
Arthrospira platensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.46
linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arthrospira platensis
-
gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.46
Arthrospira platensis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.46
linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733180
Arthrospira platensis
gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.46
?
x * 47000, SDS-PAGE
Arthrospira platensis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.46
Ferredoxin
-
Arthrospira platensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.46
expressed in Escherichia coli DH5alpha cells
Arthrospira platensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.46
Ferredoxin
-
Arthrospira platensis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.46
D138N
inactive
Arthrospira platensis
1.14.19.46
E140Q
inactive
Arthrospira platensis
1.14.19.46
G136H
inactive
Arthrospira platensis
1.14.19.46
H124R
inactive
Arthrospira platensis
1.14.19.46
H128R
inactive
Arthrospira platensis
1.14.19.46
H129R
inactive
Arthrospira platensis
1.14.19.46
H305R
the mutant shows 17% of wild type activity
Arthrospira platensis
1.14.19.46
H306R
inactive
Arthrospira platensis
1.14.19.46
H313R
inactive
Arthrospira platensis
1.14.19.46
H315N
inactive
Arthrospira platensis
1.14.19.46
H89R
inactive
Arthrospira platensis
1.14.19.46
H93R
the mutant shows 11% of wild type activity
Arthrospira platensis
1.14.19.46
R123N
the mutant shows 91% of wild type activity
Arthrospira platensis
1.14.19.46
W294G
inactive
Arthrospira platensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.19.46
0.5
-
linoleic acid
mutant enzyme R123N, at pH 7.5 and 25°C; wild type enzyme, at pH 7.5 and 25°C
Arthrospira platensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.46
47000
-
x * 47000, SDS-PAGE
Arthrospira platensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.46
linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arthrospira platensis
-
gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.46
linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733180
Arthrospira platensis
gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.46
?
x * 47000, SDS-PAGE
Arthrospira platensis