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Literature summary extracted from

  • Chazarreta Cifre, L.; Alemany, M.; de Mendoza, D.; Altabe, S.
    Exploring the biosynthesis of unsaturated fatty acids in Bacillus cereus ATCC 14579 and functional characterization of novel acyl-lipid desaturases (2013), Appl. Environ. Microbiol., 79, 6271-6279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.19 expressed in Bacillus subtilis strain LSC0400 Bacillus cereus
1.14.19.45 expressed in Bacillus subtilis Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.19 membrane
-
 Bacillus cereus 16020
-
1.14.19.45 membrane
-
 Bacillus cereus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.45 39805
-
 x * 39805, calculated from amino acid sequence Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.19 (4E,8E)-sphinga-4,8-dienine ceramide + flavodoxin + O2 + H+ Bacillus cereus
-
 ?
-
 ?
1.14.19.19 (4E,8E)-sphinga-4,8-dienine ceramide + flavodoxin + O2 + H+ Bacillus cereus ATCC 14579
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.19 Bacillus cereus
-
-
-
1.14.19.19 Bacillus cereus ATCC 14579
-
-
-
1.14.19.45 Bacillus cereus
-
-
-
1.14.19.45 Bacillus cereus ATCC 14579
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.19 (4E,8E)-sphinga-4,8-dienine ceramide + flavodoxin + O2 + H+
-
 Bacillus cereus ?
-
 ?
1.14.19.19 (4E,8E)-sphinga-4,8-dienine ceramide + flavodoxin + O2 + H+
-
 Bacillus cereus ATCC 14579 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.45 ? x * 39805, calculated from amino acid sequence Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
1.14.19.19 DELTA10 desaturase
-
 Bacillus cereus
1.14.19.19 DesB
-
 Bacillus cereus
1.14.19.45 DELTA5 Des
-
 Bacillus cereus
1.14.19.45 DELTA5 desaturase
-
 Bacillus cereus
1.14.19.45 DesA
-
 Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.19 flavodoxin YkuN
-
 Bacillus cereus
1.14.19.19 flavodoxin YkuP
-
 Bacillus cereus
1.14.19.45 Ferredoxin
-
 Bacillus cereus
1.14.19.45 flavodoxin
-
 Bacillus cereus

Expression

EC Number Organism Comment Expression
1.14.19.19 Bacillus cereus the expression or the activities of the enzyme is upregulated by a downshift in growth temperature to 25°C up