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Literature summary extracted from

  • Lonvaud-Funel, A.; de Saad, A.M.
    Purification and properties of a malolactic enzyme from a strain of Leuconostoc mesenteroides isolated from grapes (1982), Appl. Environ. Microbiol., 43, 357-361.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.101 citrate competitive Leuconostoc mesenteroides
4.1.1.101 D-fructose-1,6-bisphosphate noncompetitive Leuconostoc mesenteroides
4.1.1.101 D-Tartrate competitive Leuconostoc mesenteroides
4.1.1.101 L-lactate noncompetitive Leuconostoc mesenteroides
4.1.1.101 L-Tartrate competitive Leuconostoc mesenteroides
4.1.1.101 meso-tartrate competitive Leuconostoc mesenteroides
4.1.1.101 oxamate noncompetitive Leuconostoc mesenteroides
4.1.1.101 succinate competitive Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.101 16.7
-
 L-malate pH 6.0, 25°C Leuconostoc mesenteroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.101 Mn2+ allosteric activator, binds to the protein before and independently of malate Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.101 235000
-
 gel filtration Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.101 Leuconostoc mesenteroides
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.101 16.23
-
 pH 6.0, 25°C Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.101 L-malate
-
 Leuconostoc mesenteroides L-lactate + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.101 5.8
-
-
 Leuconostoc mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.101 NAD+ Km value 0.045 mM Leuconostoc mesenteroides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.101 0.000011
-
 citrate pH 6.0, 25°C Leuconostoc mesenteroides
4.1.1.101 1.2
-
 L-Tartrate pH 6.0, 25°C Leuconostoc mesenteroides
4.1.1.101 9
-
 oxamate pH 6.0, 25°C Leuconostoc mesenteroides
4.1.1.101 10
-
 meso-tartrate pH 6.0, 25°C Leuconostoc mesenteroides
4.1.1.101 11
-
 D-Tartrate pH 6.0, 25°C Leuconostoc mesenteroides
4.1.1.101 32
-
 D-fructose-1,6-bisphosphate pH 6.0, 25°C Leuconostoc mesenteroides
4.1.1.101 78
-
 succinate pH 6.0, 25°C Leuconostoc mesenteroides
4.1.1.101 276
-
 L-lactate pH 6.0, 25°C Leuconostoc mesenteroides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.101 Leuconostoc mesenteroides isoelectric focusing
-
 4.35