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Literature summary extracted from

  • Singh, P.; Banik, R.M.
    Biochemical characterization and antitumor study of L-glutaminase from Bacillus cereus MTCC 1305 (2013), Appl. Biochem. Biotechnol., 171, 522-531.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.2 2-mercaptoethanol 138.22% activity at 100 mM Bacillus cereus
3.5.1.2 cysteine 128.15% activity at 100 mM Bacillus cereus
3.5.1.2 dithiothreitol 125.13% activity at 100 mM Bacillus cereus
3.5.1.2 glutathione 129.32% activity at 100 mM Bacillus cereus
3.5.1.2 L-ascorbic acid 128.23% activity at 100 mM Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 Ca2+ 72.59% residual activity at 100 mM Bacillus cereus
3.5.1.2 Cd2+ complete inhibition at 50 mM Bacillus cereus
3.5.1.2 Co2+ 70.53% residual activity at 100 mM Bacillus cereus
3.5.1.2 Cu2+ complete inhibition at 50 mM Bacillus cereus
3.5.1.2 Hg2+ complete inhibition at 50 mM Bacillus cereus
3.5.1.2 iodoacetamide complete inhibition at 50 mM Bacillus cereus
3.5.1.2 Mg2+ 74.29% residual activity at 100 mM Bacillus cereus
3.5.1.2 Mn2+ 76.23% residual activity at 100 mM Bacillus cereus
3.5.1.2 additional information not inhibited by EDTA Bacillus cereus
3.5.1.2 p-chloromercuribenzoic acid complete inhibition at 10 mM Bacillus cereus
3.5.1.2 Pb2+ 69.27% residual activity at 100 mM Bacillus cereus
3.5.1.2 Zn2+ 82.12% residual activity at 100 mM Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 6.25
-
 L-glutamine at pH 7.5 and 35°C Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 K+ 139.43% activity at 100 mM Bacillus cereus
3.5.1.2 Na+ 120.35% activity at 100 mM Bacillus cereus
3.5.1.2 PO43- 143.13% activity at 100 mM Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 35000
-
 4 * 35000, SDS-PAGE Bacillus cereus
3.5.1.2 140000
-
 gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-glutamine + H2O Bacillus cereus
-
 L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O Bacillus cereus MTCC 1305
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Bacillus cereus
-
-
-
3.5.1.2 Bacillus cereus MTCC 1305
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 DEAE-cellulose column chromatography Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 0.502
-
 crude extract, at pH 7.5 and 35°C Bacillus cereus
3.5.1.2 7.44
-
 after 14.8fold purification, at pH 7.5 and 35°C Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 D-glutamine + H2O 48.5% activity compared to L-glutamine Bacillus cereus D-glutamate + NH3
-
 ?
3.5.1.2 D-glutamine + H2O 48.5% activity compared to L-glutamine Bacillus cereus MTCC 1305 D-glutamate + NH3
-
 ?
3.5.1.2 L-glutamic acid + H2O 13.5% activity compared to L-glutamine Bacillus cereus ?
-
 ?
3.5.1.2 L-glutamic acid + H2O 13.5% activity compared to L-glutamine Bacillus cereus MTCC 1305 ?
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Bacillus cereus L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O 100% activity Bacillus cereus L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Bacillus cereus MTCC 1305 L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O 100% activity Bacillus cereus MTCC 1305 L-glutamate + NH3
-
 ?
3.5.1.2 additional information the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide Bacillus cereus ?
-
 ?
3.5.1.2 additional information the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide Bacillus cereus MTCC 1305 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 homotetramer 4 * 35000, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 L-glutaminase
-
 Bacillus cereus
3.5.1.2 L-glutamine amidohydrolase
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 35
-
-
 Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.2 30 45 the enzyme shows relative activity above 70% for temperature range from 30 to 45°C and inactivation of enzyme at higher temperature above 50°C Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.2 20 50 the enzyme retains relative stability up to 95% from 20 to 35°C and 50% from 40 to 50°C for 30 min. The stability of enzyme decreases sharply at higher temperature Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.2 0.222
-
 L-glutamine at pH 7.5 and 35°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 7.5
-
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.2 7 8 more than 70% relative activity in pH range from 7.0 to 8.0. Loss of activity is elevated at higher pH (above 8.0), and the enzyme retains 27% of optimum activity when assayed at pH 8.5 Bacillus cereus