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Literature summary extracted from
- Biochemical characterization of the POM-1 metallo-beta-lactamase from Pseudomonas otitidis (2015), Antimicrob. Agents Chemother., 59, 1755-1758.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.2.6 | recombinant overexpression in Escherichia coli strain BL21(DE3) | Pseudomonas otitidis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.2.6 | additional information | - |
additional information | steady-state kinetics | Pseudomonas otitidis | |
| 3.5.2.6 | 0.004 | - |
ertapenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis |  |
| 3.5.2.6 | 0.007 | - |
cephalothin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.007 | - |
doripenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.016 | - |
meropenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.023 | - |
ampicillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.052 | - |
Imipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.053 | - |
piperacillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.063 | - |
cefotaxime | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.102 | - |
panipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 0.5 | - |
ticarcillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.6 | Zn2+ | a dizinc enzyme | Pseudomonas otitidis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 28636 | - |
4 * 28636, recombinant enzyme, mass spectrometry, 4 * 28639, sequence calculation | Pseudomonas otitidis |
| 3.5.2.6 | 28639 | - |
4 * 28636, recombinant enzyme, mass spectrometry, 4 * 28639, sequence calculation | Pseudomonas otitidis |
| 3.5.2.6 | 115000 | - |
recombinant enzyme, gel filtration | Pseudomonas otitidis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.6 | Pseudomonas otitidis | E6Y3G9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.2.6 | recombinant enzyme from Escherichia coli strain BL21(DE3) by ultrafiltration and anion exchange chromatography | Pseudomonas otitidis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.6 | ampicillin + H2O | - |
Pseudomonas otitidis | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | cefotaxime + H2O | - |
Pseudomonas otitidis | ? | - |
? | |
| 3.5.2.6 | cephalothin + H2O | - |
Pseudomonas otitidis | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | doripenem + H2O | - |
Pseudomonas otitidis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-([(3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | ertapenem + H2O | - |
Pseudomonas otitidis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl]sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | imipenem + H2O | - |
Pseudomonas otitidis | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([2-[(iminomethyl)amino]ethyl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | meropenem + H2O | - |
Pseudomonas otitidis | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | additional information | the recombinant enzyme shows broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins. Poor activity with ceftazidime and cefepime | Pseudomonas otitidis | ? | - |
? | |
| 3.5.2.6 | panipenem + H2O | - |
Pseudomonas otitidis | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S)-1-ethanimidoylpyrrolidin-3-yl]sulfanyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| 3.5.2.6 | piperacillin + H2O | - |
Pseudomonas otitidis | (2R,4S)-2-[(R)-carboxy([(2R)-2-[(4-ethyl-2,3-dioxopiperazine-1-carbonyl)amino]-2-phenylacetyl]amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| 3.5.2.6 | ticarcillin + H2O | - |
Pseudomonas otitidis | (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.2.6 | tetramer | 4 * 28636, recombinant enzyme, mass spectrometry, 4 * 28639, sequence calculation | Pseudomonas otitidis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.2.6 | metallo-beta-lactamase | - |
Pseudomonas otitidis |
| 3.5.2.6 | POM-1 | - |
Pseudomonas otitidis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.6 | 30 | - |
assay at | Pseudomonas otitidis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.2.6 | 9 | - |
piperacillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 51 | - |
doripenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 56 | - |
cefotaxime | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 60 | - |
cephalothin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 69 | - |
ertapenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 190 | - |
ampicillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 190 | - |
ticarcillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 220 | - |
meropenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 312 | - |
Imipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 500 | - |
panipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.2.6 | evolution | the POM-1 metallo-beta-lactamase is a subclass B3 resident enzyme | Pseudomonas otitidis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.2.6 | 170 | - |
piperacillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 390 | - |
ticarcillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 900 | - |
cefotaxime | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 4900 | - |
panipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 6000 | - |
Imipenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 6800 | - |
doripenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 8100 | - |
ampicillin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 8900 | - |
cephalothin | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 14000 | - |
meropenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
| 3.5.2.6 | 15000 | - |
ertapenem | pH not specified in the publication, 30°C, recombinant enzyme | Pseudomonas otitidis | |
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