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Literature summary extracted from

  • Wei, G.; Tian, N.; Valery, A.; Zhong, Y.; Schuppan, D.; Helmerhorst, E.
    Identification of pseudolysin (lasB) as an aciduric gluten-degrading enzyme with high therapeutic potential for celiac disease (2015), Am. J. Gastroenterol., 110, 899-908.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.24.26 nutrition pseudolysin is an enzyme cleaving gluten effectively at extremely low as well as near neutral pH values. The potential to degrade gluten during gastric transport opens possibilities for its application as a therapeutic agent for the treatment of celiac disease Acinetobacter junii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.26 extracellular
-
 Acinetobacter junii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.26 Zn2+ a zinc metalloprotease Acinetobacter junii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.26 33000
-
 x * 33000, mature enzyme, SDS-PAGE Acinetobacter junii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.26 casein + H2O Acinetobacter junii
-
 ?
-
 ?
3.4.24.26 casein + H2O Acinetobacter junii FA10
-
 ?
-
 ?
3.4.24.26 Gliadin + H2O Acinetobacter junii
-
 ?
-
 ?
3.4.24.26 Gliadin + H2O Acinetobacter junii FA10
-
 ?
-
 ?
3.4.24.26 gluten + H2O Acinetobacter junii
-
 ?
-
 ?
3.4.24.26 gluten + H2O Acinetobacter junii FA10
-
 ?
-
 ?
3.4.24.26 additional information Acinetobacter junii pseudolysin eliminates epitopes recognized by the R5 antibody, while those detected by the G12 antibody remain intact, despite destruction of the nearby major T-cell epitope QPQLPY ?
-
 ?
3.4.24.26 additional information Acinetobacter junii FA10 pseudolysin eliminates epitopes recognized by the R5 antibody, while those detected by the G12 antibody remain intact, despite destruction of the nearby major T-cell epitope QPQLPY ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Acinetobacter junii
-
 gene lasB
-
3.4.24.26 Acinetobacter junii FA10
-
 gene lasB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26 native extracellular enzyme from culture supernatant by anion exchange chromatography and ultrafiltration Acinetobacter junii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 casein + H2O
-
 Acinetobacter junii ?
-
 ?
3.4.24.26 casein + H2O
-
 Acinetobacter junii FA10 ?
-
 ?
3.4.24.26 Gliadin + H2O
-
 Acinetobacter junii ?
-
 ?
3.4.24.26 Gliadin + H2O
-
 Acinetobacter junii FA10 ?
-
 ?
3.4.24.26 gluten + H2O
-
 Acinetobacter junii ?
-
 ?
3.4.24.26 gluten + H2O
-
 Acinetobacter junii FA10 ?
-
 ?
3.4.24.26 additional information pseudolysin eliminates epitopes recognized by the R5 antibody, while those detected by the G12 antibody remain intact, despite destruction of the nearby major T-cell epitope QPQLPY Acinetobacter junii ?
-
 ?
3.4.24.26 additional information pseudolysin eliminates epitopes recognized by the R5 antibody, while those detected by the G12 antibody remain intact, despite destruction of the nearby major T-cell epitope QPQLPY Acinetobacter junii FA10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.26 ? x * 33000, mature enzyme, SDS-PAGE Acinetobacter junii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.26 37
-
 assay at Acinetobacter junii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.26 4 7 no activity at pH 2.0, the enzyme can resist and be active at low pH values e.g. in the human stomach Acinetobacter junii

General Information

EC Number General Information Comment Organism
3.4.24.26 evolution pseudolysin is a metalloprotease belonging to the thermolysin (M4) family proteases Acinetobacter junii