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Literature summary extracted from

  • Tishchenko, S.; Gabdulkhakov, A.; Tin, U.; Kostareva, O.; Lin, C.; Katanaev, V.L.
    Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Galphao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036 (2013), Acta Crystallogr. Sect. F, 69, 61-64.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.1 recombinant expression of His6-tagged truncation enzyme mutants GalphaoDELTA21 and GalphaoDELTA109 in Escherichia coli strain M15 Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.1 Galphao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036, method screening, hanging-drop vapour-diffusion method, mixing of 0.0015 ml of 25 mg/ml protein solution containing 20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 150 mM NaCl, 2 mM DTT, with 0.0015 ml of reservoir solution containing 20% PEG 4000, 100 mM HEPES sodium salt, pH 7.5, and equilibration against 0.3 ml of reservoir solution, 8-10 days, 22°C, X-ray diffraction structure determination and analysis at 2.0 A resolution, model fitting and refinement Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.5.1 20000
-
 x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE Drosophila melanogaster
3.6.5.1 38000
-
 x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE Drosophila melanogaster
3.6.5.1 58000
-
 x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.1 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.1 recombinant His6-tagged truncation enzyme mutants GalphaoDELTA21 and GalphaoDELTA109 from Escherichia coli strain M15 by nickel affinity chromatography, for the first followed by anion exchange chromatography and gel filtration Drosophila melanogaster

Subunits

EC Number Subunits Comment Organism
3.6.5.1 ? x * 58000, recombinant Galphao-CG5036 complex, SDS-PAGE, x * 38000, recombinant enzyme subunit mutant GalphaoDELTA21, SDS-PAGE, x * 20000, recombinant mutant GalphaoDELTA109, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.6.5.1 Galphao
-
 Drosophila melanogaster