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Literature summary extracted from

  • Reinhard, L.; Mueller-Dieckmann, J.; Weiss, M.S.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis (2012), Acta Crystallogr. Sect. F, 68, 1089-1093.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.18 gene dape, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) chaperone combination 3 (cc3) cells, which co-express the chaperones GroEL and GroES in order to increase the yield of soluble protein expression Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.18 purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.001-0.002 ml of 3-7 mg/ml protein in 50 mM Bis-Tris, pH 6.0, 200 mM NaCl, 0.5 mM TCEP is mixed with 0.001-0.002 ml reservoir solution, containing 5-10% w/v PEG 4000 or PEG 3350, 35-120 mM ammonium sulfate, 100 mM sodium acetate, pH 4.1-4.6, and equilibrated against 0.9 ml reservoir solution, at room temperature, 1 day, method optimization, X-ray diffraction structure determination and analysis at 2.4-2.58 A resolution, two crystal forms Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.18 N-succinyl-LL-2,6-diaminoheptanedioate + H2O Mycobacterium tuberculosis
-
 succinate + LL-2,6-diaminoheptanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.18 Mycobacterium tuberculosis P9WHS9 gene dapE or Rv1202
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.18 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.18 N-succinyl-LL-2,6-diaminoheptanedioate + H2O
-
 Mycobacterium tuberculosis succinate + LL-2,6-diaminoheptanedioate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.18 DapE
-
 Mycobacterium tuberculosis
3.5.1.18 S-DAP deacylase
-
 Mycobacterium tuberculosis
3.5.1.18 succinyl-diaminopimelate desuccinylase
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.5.1.18 metabolism the enzyme catalyzes the seventh reaction step of the succinyl pathway Mycobacterium tuberculosis