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Literature summary extracted from

  • Maize, K.; Kurbanov, E.; De La Mora-Rey, T.; Geders, T.; Hwang, D.; Walters, M.; Johnson, R.; Amin, E.; Finzel, B.
    Anthrax toxin lethal factor domain 3 is highly mobile and responsive to ligand binding (2014), Acta Crystallogr. Sect. D, 70, 2813-2822.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.83
-
 Bacillus anthracis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.83 in complex with inhibitors N-2-benzyl-N-2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-D-alaninamide, N2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-N-2-(4-nitrobenzyl)-D-alaninamide, N-2-[4-(aminomethyl)benzyl]-N-2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-D-alaninamide to 2.2-2.5 A resolution. Identification of frequently populated conformational states termed bioactive, open and tight. The bioactive position is observed with large substrate peptides and leaves all peptide-recognition subsites open and accessible. The tight state is seen in unliganded and small-molecule complex structures. In this state, domain 3 is clamped over certain substrate subsites, blocking access. The open position appears to be an intermediate state between these extremes and is observed owing to steric constraints imposed by specific bound ligands Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.83 N-2-benzyl-N-2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-D-alaninamide
-
 Bacillus anthracis
3.4.24.83 N-2-[4-(aminomethyl)benzyl]-N-2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-D-alaninamide
-
 Bacillus anthracis
3.4.24.83 N2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-N-2-(4-nitrobenzyl)-D-alaninamide
-
 Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.83 Bacillus anthracis P15917
-
-

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.24.83 0.0056
-
 pH not specified in the publication, temperature not specified in the publication Bacillus anthracis N-2-[4-(aminomethyl)benzyl]-N-2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-D-alaninamide
3.4.24.83 0.0149
-
 pH not specified in the publication, temperature not specified in the publication Bacillus anthracis N2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-N-2-(4-nitrobenzyl)-D-alaninamide
3.4.24.83 0.0152
-
 pH not specified in the publication, temperature not specified in the publication Bacillus anthracis N-2-benzyl-N-2-[(4-fluoro-3-methylphenyl)sulfonyl]-N-hydroxy-D-alaninamide