Literature summary extracted from

Begum, A.; Drebes, J.; Kikhney, A.; Mueller, I.B.; Perbandt, M.; Svergun, D.; Wrenger, C.; Betzel, C.
Staphylococcus aureus thiaminase II: oligomerization warrants proteolytic protection against serine proteases (2013), Acta Crystallogr. Sect. D, 69, 2320-2329.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.99.2	expressed in Escherichia coli BLR (DE3) cells	Staphylococcus aureus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.5.99.2	hanging drop vapor diffusion method	Staphylococcus aureus

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.5.99.2	D111A/K115A	the activity of the monomeric mutant is not significantly altered in comparison to the wild type enzyme	Staphylococcus aureus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.5.99.2	1.4	-	thiamine	wild type enzyme, at pH 7.5 and 37°C	Staphylococcus aureus
3.5.99.2	2.25	-	thiamine	mutant enzyme D111A/K115A, at pH 7.5 and 37°C	Staphylococcus aureus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.99.2	24000	-	4 * 24000, SDS-PAGE	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.5.99.2	4-amino-5-aminomethyl-2-methylpyrimidine + H2O	Staphylococcus aureus	-	4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3	-	?
3.5.99.2	thiamine + H2O	Staphylococcus aureus	-	4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.99.2	Staphylococcus aureus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.99.2	Strep-Tactin Sepharose column chromatography and Superdex 200 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.99.2	4-amino-5-aminomethyl-2-methylpyrimidine + H2O	-	Staphylococcus aureus	4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3	-	?
3.5.99.2	thiamine + H2O	-	Staphylococcus aureus	4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.5.99.2	homotetramer	4 * 24000, SDS-PAGE	Staphylococcus aureus

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.99.2	TenA	-	Staphylococcus aureus
3.5.99.2	thiaminase II	-	Staphylococcus aureus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.5.99.2	0.00407	-	thiamine	wild type enzyme, at pH 7.5 and 37°C	Staphylococcus aureus
3.5.99.2	0.0068	-	thiamine	mutant enzyme D111A/K115A, at pH 7.5 and 37°C	Staphylococcus aureus

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.5.99.2	0.00292	-	thiamine	wild type enzyme, at pH 7.5 and 37°C	Staphylococcus aureus
3.5.99.2	0.00304	-	thiamine	mutant enzyme D111A/K115A, at pH 7.5 and 37°C	Staphylococcus aureus

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Release 2023.1 (January 2023)