Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Smith, E.T.; Johnson, D.A.
    Human enteropeptidase light chain: bioengineering of recombinants and kinetic investigations of structure and function (2013), Protein Sci., 22, 577-585.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.9 expressed in Pichia pastoris X-33 strain Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.9 R96Q the mutant shows decreased specificities for substrates containing the sequences DDDDK and DDDDR, while basic pancreatic trypsin inhibitor inhibition is increased Homo sapiens
3.4.21.9 Y174R the mutant shows improved specificities for substrates containing the sequences DDDDK and DDDDR, while basic pancreatic trypsin inhibitor inhibition is significantly decreased Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.9 basic pancreatic trypsin inhibitor reversible inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.9 0.00258
-
 GD4R-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.00629
-
 GD4R-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.0179
-
 GDDDDK-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.018
-
 GD4R-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.0298
-
 GDDDDK-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.165
-
 Z-Lys-SBzl mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.172
-
 Z-Lys-SBzl mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.19
-
 Z-Lys-SBzl wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.276
-
 GDDDDK-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.9 Ca2+ CaCl2 enhances enzyme activity at 0.02 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.9 26000
-
 x * 26000, recombinant catalytic subunit, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.9 trypsinogen + H2O Homo sapiens cleavage sequence DDDDK-X trypsin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.9 Homo sapiens P98073
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.9 soybean trypsin inhibitor-agarose column chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.9 GD4R-4-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.9 GDDDDK-4-nitroanilide + H2O
-
 Homo sapiens GDDDDK + 4-nitroaniline
-
 ?
3.4.21.9 trypsinogen + H2O cleavage sequence DDDDK-X Homo sapiens trypsin + ?
-
 ?
3.4.21.9 Z-Lys-SBzl + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.9 ? x * 26000, recombinant catalytic subunit, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.9 enterokinase
-
 Homo sapiens
3.4.21.9 EPL catalytic subunit Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.9 42
-
 GD4R-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 45
-
 GD4R-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 49
-
 GD4R-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 122
-
 GDDDDK-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 135
-
 GDDDDK-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 148
-
 GDDDDK-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 319
-
 Z-Lys-SBzl mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 336
-
 Z-Lys-SBzl wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 354
-
 Z-Lys-SBzl mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.9 0.0000461
-
 basic pancreatic trypsin inhibitor wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.0000632
-
 basic pancreatic trypsin inhibitor mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 0.000228
-
 basic pancreatic trypsin inhibitor mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.9 537
-
 GDDDDK-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 1770
-
 Z-Lys-SBzl wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 1860
-
 Z-Lys-SBzl mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 2150
-
 Z-Lys-SBzl mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 2330
-
 GD4R-4-nitroanilide mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 4530
-
 GDDDDK-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 6830
-
 GDDDDK-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 7200
-
 GD4R-4-nitroanilide wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens
3.4.21.9 18900
-
 GD4R-4-nitroanilide mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C Homo sapiens